|
|||||
|
|
| 应用微卫星对白念珠菌种内分型 | |
| 引用本文： | 高飞,施伟民,陈德利,沈亮亮,邸玉君,霍克克. 应用微卫星对白念珠菌种内分型[J]. 中国麻风皮肤病杂志, 2006, 22(7): 559-561 |
| 作者姓名： | 高飞 施伟民 陈德利 沈亮亮 邸玉君 霍克克 |
| 作者单位： | 1. 云南省第一人民医院皮肤科,昆明,650032 2. 同济大学附属同济医院皮肤科 3. 复旦大学基因工程国家重点实验室 |
| 摘 要： | 目的:用rDNA以外的重复序列(微卫星)对白念珠菌进行种内分型。方法:选取临床分离株20株以及标准株1株,设计针对保守基因CDC3的引物进行PCR,PCR产物连接到载体,转化,然后测序和分型。结果:21株菌株依微卫星型别被分为5种基因型,依片段长度型别分为7型。2株临床分离株和1株标准株经转种10次以后基因型仍然保持稳定。结论:CDC3中的微卫星遗传稳定,可以作为基因分型标记。用该方法可以进行白念珠菌种内分型,将为开展白念珠菌病流行病学调查提供一个方法。 |
| 关 键 词： | 白念珠菌 基因型 微卫星 |
| 收稿时间： | 2005-09-13 |
| 修稿时间： | 2006-01-09 |
Study on the genotyping of Candida albicans by microsatellite assay |
|
| Gao Fei, Shi Weimin, Chert Deli,et al.. Study on the genotyping of Candida albicans by microsatellite assay[J]. China Journal of Leprosy and Skin Diseases, 2006, 22(7): 559-561 | |
| Authors: | Gao Fei Shi Weimin Chert Deli et al. |
| Affiliation: | Department of Dermatology and Venereology , Yunnan First People Hospital, Kanming, 650032 |
| Abstract: | Objective: To find a way for strain identification of Candida albicans(C.albicans) by repeat sequence(microsatellite),which came from other gene except for rDNA.Methods: Twenty strains were sampled from outpatient and 1 standard strain was selected.By using the primer designed for CDC3 PCR was performed,cloning the amplification products and analyzing them after auto-sequencing.Results: Five genotypes by microsatellite and 7 genotypes by length of amplification products were differentiated among 21 strains.The genotype of 3 strains kept stable after subculture 10 times.(Conclusion): The microsatellite inheritance of CDC3 is stable,which can be used for molecular marker and provide a method to study epidemiology of C.albicans. |
| Keywords: | Candida albicans genotype microsatellite |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |