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Generation of immunoglobulin secreting cells in mixed lymphocyte culture
Authors:M R Garovoy  M A Reddish  A K Abbas
Affiliation:1. Histocompatibility Laboratories of the Puget Sound Blood Center, the Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington, USA;2. Division of Medical Oncology, Department of Medicine, University of Washington, Seattle, Washington, USA
Abstract:The nature of the cellular interactions and role of the HLA system in the generation of immunoglobulin secreting cells in primary and secondary mixed lymphocyte cultures were investigated. The B lymphocyte response to alloantigen stimulation as measured by a Protein A reverse hemolytic plaque assay, consisted of polyclonal activation with production of IgG, IgM, IgA secreting cells detectable as early as day 4 in a primary and by 24 hr in a secondary mixed lymphocyte culture. B cell activation was shown to be dependent upon collaboration with T helper cells. A disparity at the HLA D/DR region between responding and stimulating cell populations was required for the induction of T helper cells. However, once activated, T helper cells could collaborate with autologous or allogeneic B lymphocytes and, without additional antigen, trigger immunoglobulin production. The mixed lymphocyte culture may now be considered a model of B cell as well as T cell activation.
Keywords:ATG  antithymocyte globulin  CY  cyclophosphamide  DNV  double normalized value  GVHD  graft-versus-host disease  HTC  homozygous typing cell  MLC  mixed lymphocyte culture  MTX  Methotrexate  RR  relative response  UPN  unique patient number
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