| 足月新生儿缺氧缺血性脑损伤大鼠模型的制作与鉴定 |
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| 引用本文： | 李丽华,屈艺,刘红卫,张莉,毛萌,母得志. 足月新生儿缺氧缺血性脑损伤大鼠模型的制作与鉴定[J]. 实验动物与比较医学, 2009, 29(4): 220-222,227. DOI: 10.3969/j.issn.1674-5817.2009.04.003 |
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| 作者姓名： | 李丽华 屈艺 刘红卫 张莉 毛萌 母得志 |
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| 作者单位： | 军事医学科学院实验动物中心,北京,100071 |
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| 基金项目： | 国家自然科学基金，中国杰出青年基金? |
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| 摘 要： | 目的检测狂犬病毒NP蛋白的免疫原性。方法利用RT-PCR扩增狂犬病毒核蛋白（NP）基因，测序后将其克隆到PVAXl真核表达载体上：将PVAX1-NP转染Vero细胞，进行SDS．PAGE,TLWestern—blot分析；以重组质粒PVAX1-NP对小鼠进行免疫试验。结果经酶切分析、鉴定和测序验证，得到重组表达质粒PVAX1-NP，NP蛋白在Vero细胞中获得了表达，且表达产物具有免疫学活性：免疫小鼠抗体水平显著升高。结论狂犬病毒NP蛋白具有免疫原性。
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| 关 键 词： | 狂犬病毒 NP基因 真核表达 |
Establishment of a Neonatal Rat Model of Hypoxic-Ischemia Brain Damage |
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| LI Min,YUAN Ju-fang,DING Lu-jing,YANG Li-ping,ZHANG Guang-zhou,SHAO Jun-shi,MA Lan-zhi,ZENG Lin. Establishment of a Neonatal Rat Model of Hypoxic-Ischemia Brain Damage[J]. Laboratory Animal and Comparative Medicine, 2009, 29(4): 220-222,227. DOI: 10.3969/j.issn.1674-5817.2009.04.003 |
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| Authors: | LI Min YUAN Ju-fang DING Lu-jing YANG Li-ping ZHANG Guang-zhou SHAO Jun-shi MA Lan-zhi ZENG Lin |
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| Affiliation: | (Laboratory Animal Center, Academy of Military Medical Science, Beijing 100071, China) |
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| Abstract: | Objective To detect the immunogenicity of NP gene of RV. Method NP gene of RV was amplified by RT-PCR and then cloned into plasmid pVAX1 to construct recombinant plasmid pVAX1- NP.The Vero cells were transfected with plasmid pVAX1-NE Expression of the NP protein was detected by SDS-PAGE and Western-blot assay. The mice were immuned with plasmid pVAXI-NP. Result The plasmid PVAX1-NP was obtained and the NP gene was expressed in Veto cells .The expression products had immunogenicity and the antibody level of immuned mice was higher. Conclusion The NP protein of RV had immunogenicity. |
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| Keywords: | RV NP gene Eukaryotic expression |
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