Ultrastructural and biochemical findings in brain cell cultures infected with canine distemper virus |
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Authors: | T Glaus C Griot A Richard U Althaus N Herschkowitz M Vandevelde |
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Institution: | (1) Institute of Animal Neurology, University of Berne, P. O. Box 2735, CH-3001 Berne, Switzerland;(2) Department of Pediatrics, School of Medicine, University of Berne, CH-3011 Berne, Switzerland |
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Abstract: | Summary To study the pathomechanism of demyelination in canine distemper (CD), dog brain cell cultures were infected with virulent A75/17-CD virus (CDV) and examined ultrastructurally. Special attention was paid to the oligodendrocytes, which were specifically immunolabelled. In addition, cerebroside sulfotransferase (CST), an enzyme specific for oligodendrocyte activity was assayed during the course of the infection. Infection and maturation as well as CDV-induced changes were found in astrocytes and brain macrophages. Infection of oligodendrocytes was rarely seen, although CST activity of the culture markedly decreased and vacuolar degeneration of these cells occurred, resulting in their complete disappearance. We concluded that the degeneration of oligodendrocytes and demyelination is not due to direct virus-oligodendrocyte interaction, but due to CDV-induced events in other glial cells.Supported by the Swiss National Science Foundation Grant nos. 3.956.87 (M. V.) and 3.156.88 (N. H.), the Swiss Multiple Sclerosis Society (M. V.) and the Swiss Foundation of Encouragement of Research in Mental Retardation (N. H.) |
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Keywords: | Brain cell culture Canine distemper virus Cerebroside sulfotransferase Electron microscopy Oligodendrocytes |
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