| 浙江沿海地区汉族人A型及AB型血型的基因亚型研究 |
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| 引用本文： | 严建新,陈敏霞. 浙江沿海地区汉族人A型及AB型血型的基因亚型研究[J]. 中国卫生检验杂志, 2010, 0(2) |
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| 作者姓名： | 严建新 陈敏霞 |
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| 作者单位： | 浙江省温岭市第一人民医院输血科; |
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| 摘 要： | 目的:探讨浙江沿海地区汉族人A型及AB型血型的基因亚型分布情况,为输血及血库血量储存提供理论依据。方法:采用序列特异引物聚合酶链反应(PCR-SSP)检测该地区汉族成人体检者血样本DNA基因亚型,A型280份,AB型110份,根据PCR-SSP反应产生的特异性阳性条带判定相应的基因亚型。结果:A型样本分型成功278份,AB型样本分型成功107份。A型血基因亚型以A/A201、A/O及A201/O较多,占97.84%,而A201/A201及A/A占2.16%;AB型血中基因亚型A/B型(51.40%)及A201/B型(48.60%)所占比例较为接近。A型及AB型血型的基因亚型分布在男女性别之间无显著性差异(P>0.05)。结论:浙江沿海地区汉族人群A型血中存在多种基因亚型,A型血型遗传多态性以及不同亚型之间的抗原性差异需进一步研究。
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| 关 键 词： | ABO血型 基因分型 序列特异引物聚合酶链反应 |
Genotype analysis of ABO blood group in Han nationality of littoral in Zhejiang |
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| Abstract: | Objective:To investigate the genotypes of the ABO blood group in the Han nationality of littoral in Zhejiang Province.Methods:Three hundred and ninety blood samples from unrelated Han nationality of littoral in Zhejiang Province including 280 case of blood group A and 110 case of blood group AB,were genotyped by the polymerase chain reaction with sequence-specific primers(PCR-SSP).Genomics DNA was extracted and amplified by four sequence specific primers,and the typing results were identified corresponding ... |
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| Keywords: | ABO blood group Genotyping Polymerase china reaction with sequence-specific primers |
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