胆囊癌组织P62蛋白的表达及其与血管生成关系

目的 :检测胆囊癌组织中P6 2蛋白、碱性成纤维细胞生长因子(bFGF)的表达和微血管密度 (MVD) ,探讨它们的相互关系 ,以及与胆囊癌临床病理特征的关系。方法 :4 1例胆囊癌和 2 2例慢性胆囊炎组织中 ,P6 2蛋白和bFGF的表达用SP免疫组化染色法进行检测。胆囊癌组织中MVD用抗CD34单抗 (mAb)做SP免疫组化染色进行检测。结果 :4 1例胆囊癌组织中 ,P6 2和bFGF表达阳性率分别为 6 3.4 %和 75 .6 % ,均高于 2 2例慢性胆囊炎组织 (P <0 .0 1)。P6 2的表达与胆囊癌淋巴结转移有关 (P <0 .0 5 ) ,与癌组织的分级、临床分期无显著关系。bFGF的表达与胆囊癌临床病理分期及组织学分级有关 (P <0 .0 5 ) ,而与淋巴结转移无显著关系。P6 2表达率与bFGF表达率呈正相关关系 (r=0 .5 2 1;P <0 .0 1)。两者的表达均与患者的年龄、性别、肿瘤的种类、是否伴有胆囊结石无关。胆囊癌组织中MVD明显高于慢性胆囊炎组织 (P <0 .0 1)。P6 2及bFGF表达阳性组织MVD高于表达阴性组织 (分别P <0 .0 5和P <0 .0 1)。MVD与胆囊癌的Nevin分期及淋巴结转移有关 (P<0 .0 1) ,与患者的年龄、性别、肿瘤种类、分化程度以及是否伴有胆囊结石无关。结论 :P6 2蛋白及bFGF的表达与胆囊癌组织中血管的生成相关 ,在胆囊癌的发生和发展过程中可能具有重

Expression of P62 in gallbladder carcinoma tissues and its correlation with angiogenesis

AIM: To detect the expressions of P62 and bFGF in human gallbladder carcinoma tissues and microvessel density(MVD) inside tumor, explore their correlation with each other, and their relationship with tumor's clinical pathological properties. METHODS: The expressions of P62 and bFGF in 41 cases of human gallbladder carcinoma tissues and 22 cases of chronic cholecystitis tissues were detected by SP immunohistochemical staining. The MVD was examined by SP immunohistochemical staining with anti CD34 mAb. RESULTS: The positive rates of P62 and bFGF in 41 cases of gallbladder carcinoma tissues (63.4% and 75.6%, respectively) were significantly higher than those in 22 cases of chronic cholecystitis tissues(P<0.01). The expression of P62 was associated with lymph node metastasis(P<0.05), but had no relation to tumor's histological grading and clinical stage. The expression of bFGF was correlated with tumor's histological grading and clinical stage, but not with lymph node metastasis. There was a positive correlation between P62 and bFGF expression rates(r = 0.512, P<0.01). Both proteins had no relation with patient's age, sex, tumor's type or cholelithiasis. MVD in the gallbladder carcinoma tissues was significant higher than that in the chronic cholecystitis tissues(P<0.01). The expression rates of P62 and bFGF were all correlated with MVD in gallbladder carcinoma tissues(P<0.05 and P<0.01), respectively. MVD in the gallbladder carcinoma tissues was associated with clinical stages and lymph node metastasis status (P<0.05, and P<0.01), respectively. but had no relation with patient's age, sex, tumor's type, histological grading and cholelithiasis. CONCLUSION: Expressions of P62 and bFGF were closely associated with angiogenesis in gallbladder carcinoma tissues, which may play a pivotal role in genesis and progression in gallbladder carcinoma. Expressions of P62 and bFGF may be helpful for diagnosis and therapy of gallbladder carcinoma.