日本血吸虫可溶性虫卵抗原38kDa分子的纯化及诊断价值的初步评估

[目的 ]探讨日本血吸虫虫卵可溶性抗原 (SEA) 38k Da分子诊断血吸虫病的应用价值。 [方法 ]用 SDS-PAGE配合电渗洗脱技术分离纯化了诊断靶抗原 SEA38k Da,并经 SDS- PAGE和 Western blot鉴定 ;将 SEA38k Da分子和 SEA用于 EL ISA法检测日本血吸虫病患者血清 ,急性期 31例、慢性期 31例 ,正常人 6 0例 ,华支睾吸虫病患者血清 30例 ,并殖吸虫病患者血清 30例。 [结果 ]SEA38k Da分子抗原和 SEA所测得的阳性率分别为90 .3%、90 .3%、1.7%、0、0和 90 .3%、83.9%、3.3%、0、0。将 SEA 38k Da分子和 SEA在 EL ISA中的 P/N比值进行比较 ,经 t检验 ,发现在检测急性血吸虫病患者 ,慢性血吸虫病患者和正常人血清时 ,两者间的 P/N比值在 3组间差异均有显著性意义 (P值均 <0 .0 0 1) ;SEA 38k Da分子在检测患者血清时的 P/N比值显著高于 SEA,而检测正常人血清时的 P/N比值显著低于 SEA。 [结论 ]纯化 SEA38k Da具有较好的敏感性和特异性 ,具有诊断潜能

PURIFICATION OF SOLUBLE EGG ANTIGEN (SEA) 38 kDa MOLECULE OF SCHISTOSOMA JAPONICUM AND PRELIMINARY EVALUATION OF ITS IMMUNODIAGNOSTIC EFFICACY

Objective] To study the applicability of soluble egg antigen (SEA) 38 kDa molecule of Schistosoma japonicum in the immunodiagnosis of schistosomiasis.[Methods] The target immunodiagnositic antigen SEA 38 kDa molecule was separated and purified by SDS PAGE and electroelution technique and evaluated by SDS PAGE and Western blotting. To compare their immunodiagnostic efficacy , the purified SEA 38 kDa and SEA were used to examine the sera from 182 cases including 31 acute schistosomiasis patients,31 chronic schistosomiasis patients,60 normal human controls,30 paragonimiasis patients and 30 clonorchiasis patients. [Results] The positive rates of purified SEA 38 kDa and SEA for acute schistosomiasis patients, chronic schistosomiasis patients, normal human controls, paragonimiasis patients and clonorchiasis patients were 90 3%, 90 3%, 1 7%, 0,0 and 90.3%,83.9%,3.3%,0,0, respectively. The P/N ratio of purified SEA 38 kDa and SEA in immu nodiagnosis of schistosomiasis by ELISA had been compared by two sample t test. It was demonstrated that there existed significant differences between purified SEA 38 kDa and SEA in the three groups of acute and chronic schistosomiasis patients and normal persons( P <0 001). The P/N ratio of SEA 38 kDa in detecting the sera from schistosomiasis patients was obviously higher than that of SEA, but when SEA 38 kDa was used to examine normal human sera, the result was converse.[Conclusion] The purified SEA 38 kDa has higher sensitivity and specificity and immunodiagnostic potential.