大鼠冷暴露后肺泡表面活性物质及血中红白细胞计数的变化

本研究观察了冷暴露后大鼠肺泡表面活性物质及血中红、白细胞的变化。48只SD大鼠分对照(N),冷暴露1d(CID)、冷暴露1周(C1W)及冷暴露2周(C2W)四组,每组12只。冷暴露组每天置冷室(-20±2℃)2h。结果:①冷暴露各组肺泡表面活性物质(PS)明显高于对照组(P<0.001),支气管肺泡灌洗液内细胞总数C1D组明显低于对照组(P<0.05),C2W组明显高于对照组(P<0.05)。②血中红细胞数在冷暴露后无变化,白细胞数C1D及C1W组明显低于对照组(P<0.01),且C1W及C2W组淋巴细胞相对增多,中性粒细胞相对减少。结果表明,急性冷暴露后(1d)使下呼吸道及血液免疫防御功能降低,冷暴露1周及2周后逐渐恢复,且特异免疫功能增强。冷暴露后PS增多有利于寒冷情况下增加肺顺应性,减少呼吸功能,增加肺的防御功能。

Changes of Rat Pulmonary Surfactant and Blood Cell Count after Cold Exposure

48 rats were divided into four groups. control(N);cold exposure one day(C1D); cold exposure one week(C1W);cold expos ure two weeks (C2W),Rats ofcold exposure groups were put in cold room (-20+2℃)two hours every day.The results were as follows. (1).The pulmonary surfactant of cold exposure groups was higher than that of control group (P<0.001). The cell amount in BALF of C1D group was lower than that of control group(P<0.05) (2) The eryth rocyte amount in blood didn't change after cold exposure(P>0.05). The leukocyte amount of ClD and C1W group was Lov/er than that of control group(P<0.01), the lymphocyte amount of ClW and C2W group was higher than that of control group (P<0.01) These results indicated that after cold exposure one day, the defensive ability of lung and blood were lowered,and after cold exposure one week and two weeks, the defensive ability recover.