内皮抑素基因对鼠移植性肝癌抑制作用的实验性研究

目的:探讨血管内皮抑素endostatin对体内人肝癌细胞在体内的影响。方法:将以pcDNA3.1为载体的鼠源性endostatin基因转染人肝癌细胞SMMC7721建立的裸鼠的肝癌模型。应用免疫组化和Westernblot检测endostatin的转染和表达。用免疫组化计数肿瘤血管密度(MVD)和血管内皮生长因子(VEGF)的阳性率。同时观察转基因肿瘤的生长情况。结果:可见endostatin在肝癌细胞的稳定表达和分泌,其MVD显著低于对照组(P<0.05),VEGF同对照组比较差异亦有显著性(P<0.05)。结论:en-dostatin基因对人肝癌细胞SMMC7721在裸鼠体内的生长有显著的抑制作用,endostatin能减慢肿瘤生长速度,但不能完全消除肿瘤。

Study on endostatin inhibitation of the growth of transplanted hepatic cancer in nude mice

Objective: To explore the effect of endostatin on the growth of human liver carcinoma in vivo. Methods: Mice endostatin gene was transferred into SMMC 7721 cancer established in nude mice. Immunohistochemistry and Western blot analysis were applied to examine the transfection and expression. The microvessel desity (MVD), the positive rate of vascular endothelial growth factor (VEGF) were studied using immunohistochemical methods. The growth of the cancer of the endostatin-transfected were observed. Results: Immunohistochemistry and Western blot proved endostatin expression and secretion from endostatin-transfected SMMC7721 cells, compared with negative control group, MVD and VEGF were lower (P<0.05). Conclusions: Endostatin mediated by pcDNA3.1 might inhibit the growth of human hepatic cancer SMMC7721 in vivo.