| 维生素E琥珀酸酯诱导人胃癌细胞凋亡中线粒体的作用 |
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| 引用本文： | 吴坤,李贵昌,赵艳,张晓华. 维生素E琥珀酸酯诱导人胃癌细胞凋亡中线粒体的作用[J]. 卫生研究, 2005, 34(1): 58-60 |
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| 作者姓名： | 吴坤 李贵昌 赵艳 张晓华 |
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| 作者单位： | 哈尔滨医科大学公共卫生学院,哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金资助项目(No.30371227) |
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| 摘 要： | 目的探讨在维生素E琥珀酸酯(vitaminEsuccinate,VES)诱导人胃腺癌SGC7901细胞凋亡过程中线粒体的作用。方法SGC7901细胞经5、10、20μgmlVES处理后,用荧光染色法观察线粒体跨膜电位(Δψm)改变,用WesternBlot法检测细胞质中细胞色素c的表达情况和caspase3的表达与激活。结果VES可使线粒体Δψm明显降低,有明显的剂量效应和时间效应关系。胞质中细胞色素c和caspase3表达增加,同时激活caspase3。结论VES可能是通过使线粒体通透性改变并释放细胞色素c,激活下游caspase3,从而诱导SGC7901细胞凋亡。
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| 关 键 词： | 维生素E琥珀酸酯 线粒体通透性 细胞色素c caspase-3有机锡 有机锡 |
| 文章编号： | 1000-8020(2005)01-0058-03 |
| 修稿时间： | 2004-04-23 |
Roles of mitochondria in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells |
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| Wu Kun,Li Gui-chang,Zhao Yan,Zhang Xiao-hua. Roles of mitochondria in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells[J]. Journal of hygiene research, 2005, 34(1): 58-60 |
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| Authors: | Wu Kun Li Gui-chang Zhao Yan Zhang Xiao-hua |
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| Affiliation: | Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin 150001, China. |
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| Abstract: | OBJECTIVE: In order to investigate the roles of mitochondria in vitamin E succinate (VES)-induced apoptosis in human gastric cancer SGC-7901 cells. METHODS: Mitochondrial transmembrane potential (deltapsi(m)) was observed by confocal laser scanning microscopy, while the expression of cytochrome c in cytosol and caspase-3 was measured by western blotting after the cells were treated with VES at 5, 10, 20 microg/ml. RESULTS: VES obviously decreased deltapsi(m) with dose- and time-dependent relationship, increased the expression of cytochrome c in cytosol and caspase-3 and activated caspase-3 as well. CONCLUSION: VES-induced apoptosis in SGC-7901 cells might involve mitochondrial permeability transition, release of cytochrome c and activation of caspase-3 downstream. |
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| Keywords: | vitamin E succinate mitochondrial permeability transition cytochrome c caspase-3 |
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