肿瘤标志物组合在乳腺癌防治中的应用

目的寻求乳腺癌标志物最佳组合,以提高乳腺癌诊断的准确性和灵敏度。方法采用ELISA法测定20例乳腺癌良性病变和85例乳腺癌患者治疗前血清肿瘤相关抗原(CA15-3)、癌胚抗原(CEA)、恶性肿瘤特异性生长因子(TSGF)和组织多肽特异性抗原(TPS),荧光定量PCR(FQ-PCR)检测C-erbB2拷贝数。结果乳腺癌组CA15-3、CEA、TSGF和TPS、C-erbB2,5种标志物的水平及阳性率明显高于良性病变组(P<0.05或P<0.01),在几种组合中,CA15-3、TSGF、C-erbB2三者组合在Ⅰ、Ⅱ期乳腺癌中阳性率达90.9%。结论 CA15-3、TSGF和C-erbB2联合检测是乳腺癌筛查较好的组合。

The application of combination of tumor markers for screening breast cancer

Objective To explore the best tumor marker combination in diagnosis of breast cancer and to improve the diagnostic accuracy and sensitivity.Methods The enzyme linked immunosorbent assay(ELISA) was used to detect serum cancer antigen 15-3(CA15-3),carcinoembryonic antigen(CEA),tumor specific growth factor(TSGF) and tissue polypeptide specific(TPS) in 20 patients with benign breast disease and 85 patients with breast cancer.Meanwhile the real-time fluorescent quantitative PCR(FQ-PCR) was used to detect the copies of C-erbB2. Results The levels and positive rates of CA153,CEA,TSGF,TPS and C-erbB2 in patients with breast cancer were higher,compared to those patients with benign breast disease,there were statistically significant differences(P<0.05 or P<0.01).The combination of CA15-3,TSGF, C-erbB2 has gained a higher positive rate(90.9%) than others in diagnosis of stagesⅠ,Ⅱ breast cancer.Conclusion The combined detection of CA15-3,TSGF,C-erbB2 is an effective method in screening breast cancer.