血小板保存期间血小板参数和乳酸代谢的变化

摘要 目的 探讨常温持续振荡保存血小板在保存期间血小板参数和乳酸代谢的变化规律，为临床筛选良好的保存损伤指标。方法 通过对常温保存血小板的PLT、MPV、PDW及乳酸进行系统的监测，分析保存期间血小板参数和乳酸代谢的变化趋势。结果 32份常温保存血小板保存第2、3天与第1天PLT比较无显著差异（P>0.05），保存第4、5天与第1天PLT有显著差异（P<0.005）；保存第2天与第1天MPV和PDW比较无显著差异（P>0.05），保存第3天至第5天分别与第1天MPV和PDW比较均有显著差异（P<0.005）；保存第2天至第5天分别与第1天乳酸生成量比较均存在显著性差异（P<0.001）。结论 血小板体外保存过程中，随保存时间的延长，PLT逐渐减少, MPV、PDW和乳酸生成水平逐步增高,而pH不断下降，提示血小板参数和乳酸代谢的变化可间接反映血小板的损伤情况。本文监控的指标中，以乳酸最为稳定，选择乳酸含量的监控可评估血小板贮存损伤，具有快捷、简单、经济的特点。

Study on platelet parameter and lactic acid in vitor during the storage of platelet

ABSTRACT Objective: To evaluate the damage of the PLT that level preserved by lasting shake in constant temperature in the period of preservation and to offer the evaluation to the clinical. Method: 32 packages of PLT were preserved for 5days by the way of shaking in constant temperature and measured the PLT、MPV、PDW and the lactic acid every day. Result: The counts of PLT showed significant different between the 4th, 5th and the 1st day(P<0.005), but it didn’t appear between the 2nd, 3rd and the 1st day(P>0.05);The value of MPV and PDW showed significant between the 3rd, 4th, 5th day and the 1st day(P<0.005), but it didn’t appear between the 2nd and the 1st day(P>0.05);The value of lactic acid present significant discrepancy between the 1st day and the last 4 days. Conclusion: During the period of preserving PLT, the count of PLT shows the tendency of descendent .As the period is prolong, the MPV、PDW and the level of lactic acid will become higher and higher, and the pH value gradually decrease ,it appears that the damage of PLT appears step by step. In this articles, lactic acid is the most stable value, So the damage of PLT can be evaluated by the level lactic acid and it is also quick simple and economical.