| 无花果叶中补骨脂素含量测定 |
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| 引用本文： | 郑岩,王红霞,陈随清.无花果叶中补骨脂素含量测定[J].中国民族民间医药杂志,2014(2):13-14. |
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| 作者姓名： | 郑岩 王红霞 陈随清 |
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| 作者单位： | 河南中医学院,河南郑州450008 |
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| 基金项目： | 郑州市科技攻关项目（14205113）. |
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| 摘 要： | 目的:建立无花果叶中补骨脂素含量的HPLC测定方法,为无花果叶的开发利用及质量标准的制定提供依据。方法:色谱柱为hypersil ODS(250mm×4.0mm、5μm);流动相为乙腈-水(55:45);流速为1.0mL/min;检测波长290nm;柱温32℃。结果:补骨脂素在2~10μg范围与峰面积线性关系良好(R2=0.9996),平均回收率为102.2%,RSD=1.80%。
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| 关 键 词： | 补骨脂素 无花果叶 HPLC 含量测定 |
Determination of Psoralen in Fig leaves |
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| Zheng Yan Wang Hongxia Chen Suiqing.Determination of Psoralen in Fig leaves[J].Chinese Journal of Ethnomedicine and Ethnopharmacy,2014(2):13-14. |
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| Authors: | Zheng Yan Wang Hongxia Chen Suiqing |
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| Institution: | Zheng Yan Wang Hongxia Chen Suiqing Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, China, 450008 |
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| Abstract: | Objective： To establish a HPLC method for the content determination of psoralen in Fig leaves. Methods： The analysisi was performed on phenomenex hydro C18 column （250mm ×4. 6mm, 4μm） , the mobile phase was Acetonitrile -H20 （55： 45） with a flow rate at 1. OmL/min, and detectional wavelength at 290nm under column temperature 32℃. Results： There was a good linear relationship when the sample size of bergenin was at a range of 2 ～ 10μg （R2 =0.9996）, the recovery was 102.2%, with RSD at 1.80%. Conclusion： This method was exact, simple, stable, fast and reproducible to be used for the content determination of psoralen in Fig leaves. |
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| Keywords: | Psoralen Fig leaves HPLC Content determination |
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