VE、BR对糖尿病大鼠胰组织抗氧化影响

目的　探讨维生素E(VitaminE ,VE)和胆红素 (bilirubin ,BR)对糖尿病大鼠胰组织抗氧化的影响。方法　对用链佐霉素 (streptozotocin,STZ)诱发的糖尿病大鼠持续 4周给予BR、VE或BR VE后 ,测定胰组织中的丙二醛(MDA)、一氧化氮 (NO)、超氧化物歧化酶 (SOD)和一氧化氮合酶 (NOS)的水平。结果　与正常对照组比较 ,糖尿病组大鼠的MDA含量显著增加 (P <0 0 1) ,SOD活性显著降低 (P <0 0 1)。糖尿病组、给药组 (BR组、VE组和BR VE组 )大鼠的NO含量、NOS活性均显著升高 (P <0 0 1)。与糖尿病组比较 ,给药组除SOD活性显著升高 (P <0 0 1)外 ,其它 3项指标都显著降低 (P <0 0 5 ,P <0 0 1)。结论　BR和VE通过保护胰组织的SOD活性 ,降低胰组织中的MDA、NO和NOS水平 ,从而减轻自由基对糖尿病大鼠的氧化损伤。

Effect of bilirubin and vitamin E on antioxidant in pancreas tissues of diabetic rats

Objective To study the effect of bilirubin (BR) and vitamin E (VitE) on antioxidant in pancreas tissues of diabetic rats.Methods The level of malondialdehyde (MDA),superoxide dismutase (SOD),nitric oxide (NO)and nitric oxide synthase (NOS) in the pancreas tissue was determined after supplemention of bilirubin and VitE to diabetic rats induced by streptozotocin.Results Compared with the control group,SOD activity significanctly decreased ( P <0 01),the contents of MDA and NO and the activity of NOS also significantly increased in pancreas tissue of the diabetic group rats ( P <0 05,P<0 01).The NO content and NOS activity also significantlyincreased in pancreas tissue of giving drugs group rats( P<0 05) .Compared with the diabetic group,except SOD activity significantly raised,and other three indexes were significantly fall ( P <0 05, P <0 01) after the addition of antioxidant.Conclusion BR and VitE can alleviate oxidative injury from free radicals in diabetic rats by protecting SOD activity and lowering the levels of MDA,NO and NOS in pancreas.