益气降浊汤对冠心病气虚血瘀型模型大鼠miR-126及Rho激酶表达的影响

目的观察益气降浊汤对冠心病气虚血瘀型模型大鼠血浆及心肌组织中miR-126及Rho激酶表达水平的影响。方法随机将48只Wistar大鼠分为空白组、模型组、中药组、单硝酸异山梨酯组,每组10只。除空白组外,其余组采用疲劳运动复合结扎冠脉方法建立模型。造模完成后,中药组给予益气降浊汤18 g/(kg·d)灌胃,单硝酸异山梨酯组给予单硝酸异山梨酯片6 mg/(kg·d)灌胃,模型组及空白组给予等量的生理盐水灌胃,均连续4周。实验结束后,全自动生化分析仪检测血清中肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)水平,ELISA法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、C反应蛋白(CRP)水平,HE染色观察心肌细胞组织变化,运用荧光定量PCR技术检测心肌组织中miR-126、Rho激酶表达水平。结果与空白组比较,模型组大鼠血清TNF-α、IL-6、CRP、CK、CK-MB、LDH水平及心肌组织中Rho激酶表达水平明显升高(P均<0.05),心肌组织中miR-126表达水平明显降低(P<0.05),心肌细胞坏死、排列紊乱,存在炎性浸润;与模型组比较,中药组及单硝酸异山梨酯组大鼠血清TNF-α、IL-6、CRP、CK、CK-MB、LDH水平及心肌组织中Rho激酶表达水平均明显降低(P均<0.05),心肌组织中miR-126表达水平均明显升高(P均<0.05),细胞排列较整齐,炎性浸润程度减轻。结论益气降浊汤可能通过调节miR-126及Rho激酶水平抑制炎性反应,减轻心肌损伤,对冠心病大鼠发挥一定保护作用。

Effect of Yiqi Jiangzhuo Decoction on the expression of miR-126 and Rho kinase in rats with coronary heart disease of type of Qi deficiency and blood stasis

Objective It is to observe the effect of Yiqi Jiangzhuo Decoction on the expression of miR-126 and Rho kinase in plasma and myocardial tissue of rats with coronary heart disease of type of Qi deficiency and blood stasis.Methods Forty-eight Wistar rats were randomly divided into blank group,model group,traditional Chinese medicine group,isosorbide mononitrate group,10 rats in each group.Except for the blank group,the other groups were given fatigue exercise combined with coronary artery ligation to establish the models of coronary heart disease.After modeling,the traditional Chinese medicine group was given Yiqi Jiangzhuo Decoction 18 g/(kg·d)by gavage,the isosorbide mononitrate group was given 6 mg/(kg·d)isosorbide mononitrate tablets by gavage,the model group and the blank group were given the same amount of normal saline by gavage,all the groups were treated for 4 consecutive weeks.After the experiment,the levels of serum creatine kinase(CK),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH)were detected by automatic biochemical analyzer detects,the levels of serum tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),C-reactive protein(CRP)were detected by and ELISA method,the pathological changes in myocardial cell tissue were observed by HE staining,and the expression levels of miR-126 and Rho kinase in myocardial tissue were detected by fluorescence quantitative PCR technology.Results Compared with the blank group,the levels of serum TNF-α,IL-6,CRP,CK,CK-MB,LDH and the expression of Rho kinase in myocardial tissue were significantly increased in the model group(all P<0.05),and the expression level of miR-126 in myocardial tissue was significantly reduced(P<0.05),the myocardial cells were necrotic,disordered,and inflammatory infiltrated;compared with the model group,the levels of serum TNF-α,IL-6,CRP,CK,CK-MB,LDH and the expression of Rho kinase in myocardial tissue were significantly reduced(all P<0.05),and the expression of miR-126 in myocardial tissue was significantly increased in the traditional Chinese medicine group and isosorbide mononitrate group(all P<0.05),and the cell arrangement was more ordered with less inflammatory infiltration.Conclusion Yiqi Jiangzhuo decoction can inhibit inflammatory reaction and reduce myocardial injury by regulating the levels of miR-126 and Rho kinase,thus to protect the rats with coronary heart disease.