宽胸理肺汤合三子养亲汤对COPD大鼠炎症因子、氧化应激及凋亡相关蛋白的影响

目的 观察宽胸理肺汤合三子养亲汤对慢性阻塞性肺疾病(COPD)大鼠炎症因子、氧化应激指标、凋亡相关蛋白表达的影响.方法 取7只健康雄性Wistar大鼠作为正常组,另取35只雄性Wistar大鼠采用熏香烟加气管注内毒素法建立COPD模型.将建模成功大鼠随机分成COPD组、中药低剂量组、中药中剂量组、中药高剂量组、乙酰半胱...

Effects of Kuanxiong Lifei Decoction plus Sanzi Yangqin Decoction on inflammatory factors,oxidative stress and apoptosis-related proteins in COPD rats

Objective It is to observe the effects of Kuanxiong Lifei Decoction combined with Sanzi Yangqin Decoction on inflammatory factors,oxidative stress and apoptosis related protein expression in rats with chronic obstructive pulmonary disease(COPD).Methods Seven healthy male Wistar rats were selected as the normal group,and another 35 male Wistar rats were induced by smoking cigarettes and injecting endotoxin into the air tube to establish COPD models.The successfully modeled rats were randomly divided into COPD group,low-dose Chinese medicine group,medium-dose Chinese medicine group,high-dose Chinese medicine group,and acetylcysteine group,with 7 rats in each group.The high,medium and low dose groups of Chinese medicine were given Kuanxiong Lifei Decoction plus Sanzi Yangqin Decoction at the doses of 5,2.5,1.25 g crude drug/kg respectively,and 0.8 g/kg acetylcysteine was given to the acetylcysteine group,the normal group and the COPD group were given an equal volume of 0.5%sodium carboxymethyl cellulose by gavage,all once a day,for 28 days.24 hours after the last gavage,the blood was taken from the heart,and the levels of serum inflammatory factors[interleukin-6(IL-6),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α)]and oxidative stress indexes[malondialdehyde(MDA),superoxide dismutase(SOD)]were detected by ELISA method,the pathological manifestations of rat lung tissue were observed by HE staining,the expression of Bcl-2,Bax,Caspase-3 and Caspase-9 protein were detected by Western blot method.Results Compared with the normal group,the levels of serum IL-6,IL-8,TNF-α,MDA and the protein expression of Bax,Caspase-3 and Caspase-9 in the lung tissue of the COPD group were significantly increased(all P<0.05),the level of serum SOD and Bcl-2 protein expression in lung tissue were significantly reduced(all P<0.05);HE staining showed that the small airway lumen of rats was significantly narrowed,connective tissue was proliferated,bronchial tube wall was thickened,and mucous cells was increased,part of the mucus can blocked the airway,the alveolar structure was disordered,the epithelial cells were shrunk and destroyed more or less,and neutrophils can be found in the lumen and surrounding blood vessels.Compared with the COPD group,the serum levels of IL-6,IL-8,TNF-α,MDA and the protein expression of Bax,Caspase-3,and Caspase-9 in the lung tissues of the traditional Chinese medicine groups and the acetylcysteine group were significantly reduced(all P<0.05),serum SOD level and Bcl-2 protein expression in lung tissue were significantly increased(all P<0.05);HE staining showed that airway epithelial cell shedding,inflammatory cell infiltration,goblet cell increase and formulation of emphysema were relieved in varying degree.Conclusion Kuanxiong Lifei Decoction plus Sanzi Yangqin Decoction can significantly improve the pathological changes of lung tissue in COPD rats,and its mechanism may be related to the inhibition of inflammatory factors,antioxidant stress and apoptosis.