应用血浆荧光定量聚合酶链反应诊断异基因造血干细胞移植受者巨细胞病毒感染

目的：探讨血浆荧光定量聚合酶链反应(Q—PCR)法对人巨细胞病毒(HCMV)活动性感染的诊断价值及对临床用药的指导意义。方法：收集15例患者共204份标本，应用荧光Q-PCR方法对其干细胞移植术后外周血浆HCMV-DNA进行动态监测。结果：血浆Q-PCR共检出8例患者共48份(23．52％)标本阳性；治疗有效者Q-PCR提示DNA拷贝迅速转阴，治疗耐药者Q-PCR提示DNA拷贝在停药后短期转阳或不转阴。结论：血浆Q-PCR是一种新的敏感、特异、快速的诊断方法，其HCMV-DNA的量化有助于早期诊断治疗，另对HCMV活化状态评估及药物疗效监测、减少药物滥用方面有重要的指导作用。

Diagnosis cytomegalovirus infection by plasma fluorogence quantitative PCR assay after hematopoietic stem cell transplantation

Objective:This study is to assess the usefulness of fluorogence quantitative PCR assay(Q PCR) for early detection of CMV activation after allogeneic stem cell transplantation and its guiding for anti CMV therapy.Method:Plasma fluorogence Q PCR assay is applied to monitor 15 allogeneic stem cell transplantation recipients.Result:48 samples from 8 recipient were positive for Q PCR, Timely initiation of therapy resulted in the rapid clearance of DNA viraemia but it recurrenced in short time by drug resistent.Conclusion:Fluorogence Q PCR is a suitable and reliable method for the monitoring of incipient active CMV infection and is a good method for guiding antiviral therapy.