| 尿蛋白糖链谱在糖尿病肾病诊断中的应用 |
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| 引用本文： | 邬明辉,张冬,汤力,韩秋霞,焦婷婷,蔡广研,陈香美,朱晗玉.尿蛋白糖链谱在糖尿病肾病诊断中的应用[J].中华实用诊断与治疗杂志,2019(3):263-267. |
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| 作者姓名： | 邬明辉 张冬 汤力 韩秋霞 焦婷婷 蔡广研 陈香美 朱晗玉 |
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| 作者单位： | 解放军总医院肾脏病科解放军肾脏病研究所肾脏疾病国家重点实验室国家慢性肾病临床医学研究中心北京市肾病研究重点实验室 |
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| 基金项目： | 国家重点研发计划(2016YFC1305500);国家自然科学基金(61471399;61671479);中国人民解放军保健专项科研课题(15BJZ35) |
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| 摘 要： | 目的探讨糖尿病肾病(diabetic nephropathy, DN)与非糖尿病肾病(non-diabetic renal disease, NDRD)患者尿蛋白糖基化差异,及鉴别诊断DN与NDRD的价值。方法 19例DN患者,依据估算肾小球滤过率(estimated glomerular filtration rate, eGFR)分为早期DN组eGFR>60 mL/(min·1.73 m^2)] 9例、晚期DN组eGFR≤60 mL/(min·1.73 m^2)] 10例;同期31例NDRD患者,其中膜性肾病10例、IgA肾病8例、系膜增生性肾小球肾炎6例及微小病变7例。采用凝集素芯片技术对各组尿蛋白糖链谱进行分析。结果 PWM识别的β(1,4)-linked GlcNAc寡聚体糖链相对丰度在早期DN、晚期DN组明显低于NDRD组(P<0.05);RCA120识别的β-Gal糖链相对丰度在早期DN、晚期DN组明显低于膜性肾病患者,但高于IgA肾病患者(P<0.05);GNA识别的Manα1-3Man糖链相对丰度在早期DN、晚期DN组明显低于微小病变患者(P<0.05);SNA识别的Sia2-6Gal/GalNAc糖链相对丰度在晚期DN组明显高于NDRD组(P<0.05);DSA识别的β-D-GlcNAc糖链相对丰度在晚期DN组明显高于NDRD组(P<0.05)。结论 DN与NDRD患者尿蛋白糖链谱存在明显差异,凝集素PWM识别的β(1,4)-linked GlcNAc寡聚体糖链可有效区分DN与NDRD。
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| 关 键 词： | 糖尿病肾病 非糖尿病肾病 尿蛋白糖链 尿蛋白 |
Application of glycopatterns in urinary protein in the diagnosis of diabetic nephropathy |
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| WU Minghui,ZHANG Dong,TANG Li,HAN Qiuxia,JIAO Tingting,CAI Guangyan,CHEN Xiangmei,ZHU Hanyu.Application of glycopatterns in urinary protein in the diagnosis of diabetic nephropathy[J].Journal of Chinese Practical Diagnosis and Therapy,2019(3):263-267. |
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| Authors: | WU Minghui ZHANG Dong TANG Li HAN Qiuxia JIAO Tingting CAI Guangyan CHEN Xiangmei ZHU Hanyu |
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| Institution: | (Department of Nephrology, Chinese PLA General Hospital,Chinese PLA Institute of Nephrology,State Key laboratory of Kidney Diseases ,Xatiotial Clinical Re sea rch Center for Chronic Kidney Diseases,Beijing Key Laboratory of Kidney Diseases , Beijing 100853, China) |
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| Abstract: | WU Minghui;ZHANG Dong;TANG Li;HAN Qiuxia;JIAO Tingting;CAI Guangyan;CHEN Xiangmei;ZHU Hanyu(Department of Nephrology, Chinese PLA General Hospital,Chinese PLA Institute of Nephrology,State Key laboratory of Kidney Diseases ,Xatiotial Clinical Re sea rch Center for Chronic Kidney Diseases,Beijing Key Laboratory of Kidney Diseases , Beijing 100853, China) |
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| Keywords: | diabetic nephropathy non-diabetic renal diseases glycan profile of urinary proteins urinary protein |
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