血清miR-181b及miR-210在妊娠期高血压疾病中表达及与炎性细胞因子的相关性

目的探讨妊娠期高血压疾病(hypertensive disorders in pregnancy, HDP)患者血清miR-181b、miR-210表达变化,分析其与白细胞介素(interleukin, IL)-1β、IL-6及肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)的关系。方法 HDP患者226例,其中妊娠期高血压(gestational hypertension, GH)60例(GH组)、轻度子痫前期(mild preeclampsia, MP)90例(MP组)、重度子痫前期(servere preeclampsia, SP)76例(SP组),同期健康孕妇60例为对照组。检测4组血清miR-181b mRNA、miR-210 mRNA相对表达量及IL-1β、IL-6及TNF-α水平;Pearson相关分析HDP患者血清miR-181b mRNA、miR-210 mRNA相对表达量与IL-1β、IL-6及TNF-α的相关性。结果对照组、GH、MP、SP组血清miR-181b mRNA相对表达量(1.42±0.65、5.73±1.60、10.94±4.28、17.13±8.16)、miR-210 mRNA相对表达量(0.84±0.13、3.62±1.05、4.74±1.36、7.92±2.17),IL-1β(2.60±0.84)、(11.26±2.53)、(17.65±6.24)、(23.92±9.16)ng/L]、IL-6(90.26±27.45)、(106.35±33.74)、(128.42±36.18)、(151.46±40.27)ng/L]及TNF-α(12.40±2.28)、(19.25±4.42)、(35.84±6.53)、(64.73±9.50)ng/L]水平依次增高(P<0.05);HDP患者血清miR-181b mRNA相对表达量与IL-1β、IL-6、TNF-α呈正相关(r=0.694,P<0.001;r=0.735,P<0.001;r=0.846,P<0.001)、miR-210 mRNA相对表达量与IL-1β、IL-6、TNF-α呈正相关(r=0.548,P=0.003;r=0.718,P<0.001;r=0.815,P<0.001)。结论 HDP患者miR-181b及miR-210表达上调,且上调程度与HDP病情严重程度及IL-1β、IL-6、TNF-α水平变化相关,可能参与HDP的发生、发展。

Expressions of serum miR-181b and miR-210 in hypertensive disorders in pregnancy and its correlation with inflammatory cytokines

HU Jidong;WEN Li;GUO Zhen;WANG Lili;ZHOU Huanhuan(Department of Obstetrics and Gynecology,Zhuozhou Hospital,Zhuozhou072750,China)