DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results |
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| Authors: | Heininger Alexandra Binder Marlies Ellinger Andreas Botzenhart Konrad Unertl Klaus Döring Gerd |
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| Affiliation: | Klinik für An?sthesiologie und Transfusionsmedizin, Abteilung für An?sthesiologie, Eberhard-Karls-Universit?t Tübingen, D-72074 Tübingen, Germany. |
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| Abstract: | DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerase. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used. |
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