| 白毛藤对人乳腺癌MCF-7细胞凋亡的影响 |
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| 引用本文： | 杨旭东,张杰,杨骄霞. 白毛藤对人乳腺癌MCF-7细胞凋亡的影响[J]. 中国现代中药, 2010, 12(4): 34-36 |
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| 作者姓名： | 杨旭东 张杰 杨骄霞 |
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| 作者单位： | 1. 黑龙江省牡丹江医学院,黑龙江,牡丹江,157011
2. 黑龙江省牡丹江医学院附属红旗医院,黑龙江,牡丹江,157011 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 目的:探讨Bcl-xl、p53基因在白毛藤诱导人乳腺癌MCF-7细胞凋亡中的作用。方法:不同浓度白毛藤作用于MCF-7细胞48h后,荧光显微镜观察人乳腺癌细胞MCF-7细胞凋亡情况,RT-PCR法检测Bcl-xl、p53基因表达量的变化。结果:白毛藤能诱导MCF-7细胞凋亡,并且上调p53和降低Bcl-xl表达。结论:白毛藤抑制人乳腺癌MCF-7细胞细胞增殖,促进凋亡,其机制可能与激活p53、抑制Bcl-xl表达有关。
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| 关 键 词： | 白毛藤 乳腺癌 细胞凋亡 |
| 收稿时间： | 2009-11-23 |
Effect of Solanum Lyratum Thunb on the Apoptosis of MCF-7 Human Breast Cancer Cells |
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| Yang Xudong,Zhang jie and Yang Jiaoxia. Effect of Solanum Lyratum Thunb on the Apoptosis of MCF-7 Human Breast Cancer Cells[J]. Modern Chinese Medicine, 2010, 12(4): 34-36 |
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| Authors: | Yang Xudong Zhang jie Yang Jiaoxia |
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| Affiliation: | Mudanjiang Medical College, Mudanjiang Heilongjiang 157011, China;Mudanjiang Medical College, Mudanjiang Heilongjiang 157011, China;HongQi Hospital Affiliated to Mudanjiang Medical College, Mudanjiang Heilongjiang 157011; China |
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| Abstract: | Objective: To investigate the effect of Bcl-xl and p53 gene on Human breast cancer MCF-7 cells. Methods: Human breast cancer MCF-7 cells were treated with various of Solanum lyratum Thunb , We observed cell apoptosis of MCF-7 cells by fluorescence microscope. We detected the levels of Bcl-xl and p53 gene expression in all groups by RT-PCR. Results: Solanum lyratum Thunb has obviously apoptosis-inducing effects on Human breast cancer MCF-7 cells and could effectively decrease the level of Bcl-xl gene expression and recrease the level of Bcl-xl gene expression. Conclusion: Solanum lyratum Thunb could induce apoptosis of MCF-7 cells probably by regulating Bcl-xl gene expression and regulating p53 gene expression. |
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| Keywords: | Solanum lyratum Thunb Breast cancer Apoptosis |
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