督脉配穴对染铅幼年大鼠海马一氧化氮合酶阳性细胞的保护作用

目的探讨督脉配穴对染铅幼年大鼠海马不同亚区一氧化氮合酶（NOS）变化的拮抗作用。方法将40只SD大鼠随机分为3组，即对照组、染铅组、督脉配穴组。采用反映学习记忆功能的Y迷宫法测试大鼠神经行为的改变，用NADPH-黄递酶（NADPH．d）组织化学法研究大鼠海马不同亚区NOS的活性神经元变化。结果染铅组大鼠的学习记忆能力比督脉配穴组和对照组明显下降（P〈0．05），但督脉配穴组与对照组之间差异无统计意义（P〉0．05）；组织化学实验结果显示，染铅组大鼠海马CA1区和齿状回的NOS阳性神经元明显少于督脉配穴组和对照组（P〈0．05），在CA3区无差别，督脉配穴组与对照组各亚区之间差异无统计意义（P〉0．05）。结论铅可损伤幼年大鼠学习记忆能力，其机制可能与染铅后海马各区NOS活性的不同变化有关，督脉配穴针灸治疗对铅引起的学习记忆损伤和NOS活性的变化有拮抗作用。

Protective effects of governor meridian acupuncture on the nitric oxide synthase positive cells in young lead-poisoned rat hippocampus

Objective To investigate the antagonistic effect of governor meridian acupuncture on the nitric ox- ide synthase level in young lead-exposed rat hippocampus subarea. Methods 40 SD rats were randomly divided into three groups, normal group, lead-exposed model group and governor meridian acupuncture group. Y-maze test was applied to study the effects of lead on learning and memory ability in rats; NADPH-d histochemistry was used to investigate the changes of nitric oxide synthase （NOS） in different hippocampal subareas in lead-exposed young rat. Results Compared with the control and governor meridian acupuncture group, the learning and memory ability of lead-exposed rats was significantly decreased （P〈0.05）, but there was no statistic significance between governor meridian acupuncture group and control group （P〉0.05） ; The number of NOS positive neurons on CA1 and dentate gyrus of lead-exposed young rats were significantly decreased （ P〈0.05 ）, but no significant changes in CA3 ; No difference between the control and governor meridian acupuncture groups in CA1, CA3 and dentate gyrus （ P〉0.05 ）. Conclusion Lead may damage the learning-memory ability of the young rats, which might be related to the changes of NOS in different subfields of hippocampus in lead-exposed rats, governor meridian acupuncture might play an important role in preventing lead-inducted damages.