葡萄酒中抗癌活性成分反式白藜芦醇含量及其环境因素的影响

目的：利用高效液相色谱法测定葡萄酒中抗癌活性成分反式白藜芦醇的含量，并研究其含量的环境影响因素。方法：采用高效液相色谱法，使用ZORBAX C18色谱柱（4.6 mm±250 mm，5μm），流动相为30％乙腈水溶液；流速为1 ml/min；检测波长为306 nm；柱温为室温；分离测定葡萄酒中反式白藜芦醇的含量，并研究光照、温度及密封状况对其含量的影响。结果：张裕干红葡萄酒中反式白藜芦醇的含量为（1.01±0.03）mg/L，长城干红解百纳中反式白藜芦醇的含量为（2.36±0.02）mg/L。阳光照射、温度升高及密封状况差均会导致反式白藜芦醇含量下降。结论：不同品牌的葡萄酒中反式白藜芦醇含量相差较大，且应在低温、避光和密封的条件下贮存葡萄酒。

Research on anti-cancer active component in red wine

Objective：To detect the anti-cancer component, trans-resveratrol in red wine by high performance liquid chromatography, and to determine the influence of environmental factors on its content. Methods： A method for determining trans-resveratrol in red wine by HPLC is described. The operating conditions were ZORBAX-C18 column（4.6 mm＆#215;250 mm, 5μm） at room temperature, 30%acetonitrile as mobile phase at a flow of 1ml/min and UV detection at 306 nm. Results：The content of trans-resveratrol in Changyu dry red wine and Great Wall Claret Cabernet are （1.01＆#177;0.03）mg/L and （2.36＆#177;0.02）mg/L, respectively. The results show that, sunlight, high temperature and poor seal condition can decrease the content of trans-resveratrol. Conclusion：The content of trans-resveratrol varies in different brands of red wine. Red wine should be stored in cool, dark and sealed conditions.