| 免疫表型分型结合FISH检测自然杀伤细胞淋巴瘤细胞的染色体畸变 |
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| 引用本文： | 翁立红,周俊,张颜明,顾文祥.免疫表型分型结合FISH检测自然杀伤细胞淋巴瘤细胞的染色体畸变[J].浙江大学学报(医学版),1999,28(6):253-256,267. |
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| 作者姓名： | 翁立红 周俊 张颜明 顾文祥 |
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| 作者单位： | 浙江大学医学院!浙江杭州310031 |
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| 摘 要： | 目的：检测自然杀伤(NK)细胞淋巴瘤中含有染色体畸变的肿瘤细胞的免疫表型。方法：采用免疫表型分型结合荧光原位杂交(FISH)技术，在单细胞水平检测了3倒NK细胞淋巴瘤中含有染色体畸变的肿瘤细胞的免疫表型。结果：在该3例中，克隆性的染色体畸变仅见于CD56 细胞，左CD3 细胞和CD5 细胞中束发现相应染色体畸变。结论：实验表明，NK细胞淋巴瘤中，含有染色体畸变的肿瘤细胞局限于CD56 、CD3-和CD5-的NK细胞群，NK细胞淋巴瘤可能来源于成熟的NK细胞而非T淋巴细胞。
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| 关 键 词： | 淋巴瘤，非霍奇全氏／病因学 杀伤细胞，天然 免疫表型分型，染色体畸变 原位杂交，荧光 抗原，CD56／分析 |
The Detection of Chromosome Aberrations in Natural Killer Cell Lymphoma/leuk1emia with a Combination of Immunophenotyping and FISH |
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| WENG Li hong,ZHOU Jun,ZHANG Yan ming,et al.The Detection of Chromosome Aberrations in Natural Killer Cell Lymphoma/leuk1emia with a Combination of Immunophenotyping and FISH[J].Journal of Zhejiang University(Medical Sciences),1999,28(6):253-256,267. |
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| Authors: | WENG Li hong ZHOU Jun ZHANG Yan ming |
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| Abstract: | Objective:To detect the immunophenotypes of tumor cells with chromosome aberrations in three cases of natural killer (NK) cell lymphoma/leukemia.Methods:A combination of immunophenotyping and FISH.Results:In all three cases,clonal chromosome aberrations were detected only in CD56 cells,not in CD3 or CD5 cells.Conclusion:This study indicates that chromosome aberrations in NK cell lymphoma/leukemia might be restricted to the CD56 ,CD3- and CD5- cell populations and that NK cell lymphoma/leukemia may derive from mature NK cells and not from T lymphocytes. |
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| Keywords: | Lymphoma non Hodgkin's/etiol Killer cells natural Immunophenotyping Chromosome aberrations In situ hybridization fluorescence Antigens CD56/anal |
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