| 抗衣原体单克隆抗体的制备及其生物学特性鉴定 |
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| 引用本文： | 沈建根 蔡挺 鲍建芳 董玉娥 尹秀. 抗衣原体单克隆抗体的制备及其生物学特性鉴定[J]. 浙江大学学报(医学版), 1999, 28(3): 97-100 |
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| 作者姓名： | 沈建根 蔡挺 鲍建芳 董玉娥 尹秀 |
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| 作者单位： | [1]浙江医科大学免疫学教研室 [2]浙江医科大学微生物学教研室 [3]浙江省人民医院皮肤科 |
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| 摘 要： | 目的：制备抗衣原体的单克隆抗体，为研制表原体快速检测卡作准备。方法：用沙眼衣原体和鹦鹉热衣原体分别免疫BALB／c小鼠．按常规淋巴细胞杂交瘤技术制各单克隆抗体。结果：获得抗沙眼衣原体及抗鹦鹉热衣原体单克隆抗体各3株，经检测6株单抗均具有较高的属特异性，不与其他微生物发生交叉反应。结论：本研究为进一步研制衣原体快速检测卡奠定了基础。
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| 关 键 词： | 单克隆抗体 制备 生物学特性 鉴定 沙眼衣原体 |
Preparation and Biologically Characteristic Identification of Monoclonal Antibodies against Chlamydiae |
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| Shen Jiangen,Cai Ting,Bao Jianfan,et al.. Preparation and Biologically Characteristic Identification of Monoclonal Antibodies against Chlamydiae[J]. Journal of Zhejiang University. Medical sciences, 1999, 28(3): 97-100 |
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| Authors: | Shen Jiangen Cai Ting Bao Jianfan et al. |
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| Abstract: | Objective: To aim at the developing of a rapid chlamydiae detection card through the preparation of monoclonal antibodies against chlamydiae. Methods: BALB/c mice were immunized with chlamydiae trachomatic and chlamydiae psittaci respectively and McAbs were prepared by standard lymphocyte hybridoma technique. Results: Three McAbs against C trachomatic and three against C psittaci were obtained.All the six strains mentioned above possessed a higher genus specificity and made no crossreaction to other microorganisms. Conclusion: This research has laid a foundation for the further development of a rapid chlamydiae detection card. |
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| Keywords: | Chlamydia trachomatis Chlamydia psittaci Hybridomas Antibodies monoclonal/isol Antibodies monclonal/anal Chlamydia infection/dig |
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