人类黑色素瘤相关抗原A9在早期宫颈癌中的表达研究

目的 检测人类黑色素瘤相关抗原A9(MAGE-A9)在正常宫颈和早期宫颈癌组织中的表达,探讨其在早期宫颈癌中的致病机制及临床病理特征之间的关系.方法 收集宫颈癌组织69例、宫颈上皮内瘤变(CIN)组织30例和正常宫颈组织标本20例,应用免疫组织化学SP法检测MAGE-A9的表达,应用统计学方法分析其与年龄、FIGO分期、组织病理学分级及淋巴结转移等因素间的关系.另取20例宫颈癌、15例C1N、15例正常的新鲜组织,应用实时荧光定量 PCR(RT-qPCR)法检测不同宫颈组织中MAGE-A9基因及其蛋白的表达水平.结果 MAGE-A9在正常宫颈组织不表达,CIN及宫颈癌组织中均有表达,阳性表达率分别为 5.00％、13. 33％、47. 83％,组间差异有统计学意义(P＜0.05).其在早期宫颈癌的表达与FIGO期、组织病理学分级、淋巴结转移有关,与年龄、组织类型无关.RT-qPCR结果提示,宫颈癌组织中的MAGE-A9表达量明显高于CIN及正常组织,宫颈癌组与正常组及CIN组间差异有统计学意义(P＜0. 05),而CIN与正常组织之间差异无统计学意义.结论 MAGE-A9在正常宫颈、CIN及宫颈癌组织中均有表达,阳性表达率随着疾病的进展而增高,提示在宫颈癌的发生发展中MAGE-A9具有重要作用.

Expression of human melanoma-associated antigen A9 in early cervical cancer

Objective To detect the expression of MAGE-A9 in normal cervix and early cervical cancer, and to explore the relationship between the expression of MAGE-A9 and the clinicopathological features of cervical cancer. Methods The expression of MAGE-A9 was detected by immunohistochemical SP method in 69 cases of cervical cancer, 30 cases of cervical intraepithelial neoplasia (CIN) and 20 cases of normal cervical tissues. The expression of MAGE-A9 was analyzed by statistical method. The relationship between age, FIGO stage, histopathological grade and lymph node metastasis was analyzed by statistical method. The expression of MAGE-A9 gene in another 20 cases of cervical cancer, 15 cases of CIN and 15 cases of normal cervical tissues was detected by real-time fluorescence quantitative PCR (RT-qPCR). Results The expression of MAGE-A9 was not detected in normal cervical tissues and CIN and cervical cancer tissues. The positive expression rates of MAGE-A9 were 5. 00％, 13. 33％ and 47. 83％ respectively. There was significant difference between the two groups (P ＜ 0. 05). The expression of MAGE-A9 in early cervical cancer was related to FIGO stage, histopathological grade and lymph node metastasis, but not to age and histological type. The results of q-PCR showed that the expression of MAGE-A9 in cervical carcinoma was significantly higher than that in CIN and normal tissues (P ＜ 0. 05 ), but there was no significant difference between cervical carcinoma and normal group and CIN group. There was no significant difference between the two groups. Conclusion MAGE-A9 is expressed in normal cervix, CIN and cervical carcinoma, and the positive expression rate of MAGE-A9 is increased with the progression of the disease. It suggests that MAGE-A9 plays an important role in the development of cervical cancer.