miR-7在口腔鳞状细胞癌中的表达及潜在临床意义

目的 研究miR-7在口腔鳞状细胞癌(OSCC)中的表达及潜在临床意义.方法 获取原发性OSCC患者肿瘤组织53例及正常健康组织27 例,利用qRT-PCR检测各组织中miR-7的表达,并分析其与上皮间质转化的相关性.利用质粒转染技术将含miR-7 序列片段的质粒导入OSCC细胞株SCC9中,通过建立过表达miR-7 的SCC9 细胞进一步验证miR-7与OSCC上皮间质转化的关系.结果 与健康组 织比较,miR-7 在 OSCC 肿瘤组织中的表达显著降低( P ＜0. 01);miR-7的表达与上皮间质转化过程呈显著相关性(P＜0. 001).与对照组细胞比较,过表达miR-7促进SCC9细胞E-cadherin的表达,抑制 Fibronectin 的表达( P ＜0. 01).结论 miR-7在OSCC中低表达,与上皮间质转化过程高度相关.

Expression and potential clinical significance of miR-7 in oral squamous cell carcinoma

Objective To investigate the expression and potential clinical significance of miR-7 in oral squamous cell carcinoma ( OSCC) . Methods Cancer tissues from 53 OSCC patients and healthy tissue from 27 people were collected. The levels of miR-7 in each tissue were detected by quantitative RT-PCR; the correlation between miR-7 and epithelial mesenchymal transition ( EMT) were analyzed. Plasmid of miR-7 was conducted into SCC9 cells to confirm the role of miR-7 in OSCC. Results Compared with the healthy tissue, the levels of miR-7 in OSCC tissue were decreased significantly ( P＜0. 01) ; the expression of miR-7 in OSCC tissue were highly correlated with epi-thelial mesenchymal transition ( P＜0. 001) . Compared with the control cells, overexpression of miR-7 promoted E-cadherin expression, but inhibited Fibronectin expression in SCC9 cells (P＜0. 01). Conclusion miR-7 is low expressed in OSCC, which is highly correlated with epithelial-mesenchymal transition.