荧光示踪法与染料法探测兔甲状腺前哨淋巴结有效性研究

目的 比较荧光示踪法与染料法探测兔甲状腺前哨淋巴结的有效性,为临床开展甲状腺前哨淋巴结活检提供实验依据.方法 30只实验兔共计60侧甲状腺,每侧甲状腺分别注射600 μmol· L-1的吲哚菁绿(ICG)和5 g· L-1的亚甲蓝0.02 mL.切除所探测的组织进行病理检查,分别记录两种方法检测的淋巴结数量,比较两种方法的准确率和检出率.结果 荧光探测法和亚甲蓝染料法检出兔甲状腺前哨淋巴结的准确率分别为92.7%和70.8%,检出率分别为91.7%和75.0%,差异有统计学意义(P<0.05).结论 在探测兔甲状腺前哨淋巴结方面荧光示踪法优于亚甲蓝染料法,具有较高的检出率和准确率.

The efficacy of fluorescence tracer method and dye method in detecting thyroid sentinel lymph nodes in rabbits

Objective To compare the efficacy of fluorescence tracer method and dye method in detecting thyroid sentinel lymph node in rabbit and to provide experimental evidences for clinical application of thyroid sentinel lymph node biopsy .Methods Sixty lobes ofthyroid from thirty rabbits were injected with 600 μmol L-1 indocyanine green and 0.02 mL of 5 g· L-1 methylene blue dye respectively.The detected tissues were resected for pathological examination and the amount of lymph nodes detected by two methods were sepa -rately recorded,and the accuracy and detection rate of the two methods were compared .Results The accuracy of fluorescence tracermethod and dye method in detecting thyroid sentinel lymph node in rabbit was 92.7% vs 70.8%.The detection rates of sentinel lymphnode in the two methods were 91.7% and 75.0% respectively.The difference was statistically significant (P <0.05).ConclusionsIn detecting the sentinel lymph node of rabbit thyroid ,the fluorescence tracer has higher detection rate and accuracy when compared todye method.