高效液相色谱波长切换法同时测定百效丸中6种主成分的含量

目的 建立高效液相色谱波长切换法同时测定百效丸中6种主成分(哈巴苷、安格洛苷C、哈巴俄苷、巴豆苷、和厚朴酚、厚朴酚)含量的方法.方法 采用Venusil MP C18(4.6 mm×250 mm,5.0μm)色谱柱;流动相A:乙腈-甲醇(1:4),流动相B:0.2%磷酸溶液,梯度洗脱;检测波长210 nm(哈巴苷)、280 nm(安格洛苷C、哈巴俄苷)、294 nm(巴豆苷、和厚朴酚、厚朴酚).结果 哈巴苷、安格洛苷C、哈巴俄苷、巴豆苷、和厚朴酚、厚朴酚分别在3.98~79.60 mg·L-1、2.65~53.00 mg·L-1、2.45~49.00 mg·L-1、6.91~138.20 mg·L-1、5.29~105.80 mg·L-1、9.37~187.40 mg·L-1范围内线性关系良好;平均回收率96.90%~100.05%,RSD值0.89%~1.59%;精密度和重复性良好;供试品溶液在室温条件下10 h内稳定.结论 该方法操作简便,可用于百效丸中6种主成分的同时测定.

Simultaneous determination of six main components in baixiao wan by HPLC wavelength switching method

Objective To establish an HPLC wavelength switching method for simultaneous determination of six main components (harpagide,angoroside C,harpagoside,2-hydroxyadenosine,honokiol and magnolol ) in Baixiao Wan.Methods The Venusil MP C18 (4.6 mm ×250 mm,5 μm) chromatographic column was used ,the mobile phase was acetonitrile-methanol (1:4) (A) -0.2% phos-phoric acid solution (B) with gradient elution,and the detection wavelengths were 210 nm for harpagide,280 nm for angoroside C and harpagoside,and 294 nm for 2-hydroxyadenosine,honokiol and magnolol .Results The linear relationship of harpagide ,angoroside C, harpagoside,2-hydroxyadenosine,honokiol and magnolol was good in the ranges of 3.98-79.60 mg· L-1 ,2.65-53.00 mg· L-1 ,2.45-49.00 mg· L-1 ,6.91-138.20 mg· L-1 ,5.29-105.80 mg· L-1 ,9.37-187.40 mg· L-1 ,respectively.The average recoveries were 96.90% ~100.05%,and the corresponding RSDs were 0.89% ~1.59%.The precision and repeatability were good .Test solution was stable at room temperature within 10 h.Conclusions The method is simple,and can be used for the simultaneous determination of six components in Baixiao Wan.