Th1/Th2趋化因子及其受体在大疱性类天疱疮皮损中的表达

目的 探讨 Th1 趋化因子 MIG/CXCL9、I-TAC/CX-CL11与其受体 CXCR3,Th2 趋化因子 TARC/CCL17、MDC/CCL22及其受体CCR4在大疱性类天疱疮( BP)患者发病中的作用.方法 应用免疫组化及光密度(OD)技术检测趋化因子CXCL9、CXCL11、CCL17、CCL22 及受体CXCR3 、CCR4在50例BP患者皮损及30例正常皮肤中的表达.结果 50例BP患者皮损中4种趋化因子及其受体阳性表达的OD值均高于正常皮肤.其中, BP 皮损和对照组 Th1 趋化因子CXCL9、CXCL11 及其受体 CXCR3 表达的 OD 值分别为(0.55 ± 0.09) vs (0.26 ± 0.08)、(0.38 ± 0.06) vs (0.18 ± 0.04)和(0.37 ± 0.04) vs (0.22 ± 0.02),Th2 趋化因子CCL17、CCL22及其受体CCR4表达的OD值分别为(0.42 ± 0.10) vs (0.25 ± 0.12)、(0.35 ± 0.04) vs (0.21 ± 0.03)和(0.42 ± 0.05) vs (0.24 ± 0.07),且表达差异均有统计学意义(P＜0.01).结论 Th1趋化因子CXCL9、CXCL11和Th2趋化因子CCL17、CCL22及其受体CXCR 3、CCR4可能与BP的发病相关,对探讨BP发病机制有一定的价值.

Expression of Th1/Th2 chemokines and their receptors in bullous pemphigoid lesions

Objective To study the role of Th1 chemokines MIG/CXCL9, I-TAC/CXCL11 and Th2 chemokines TARC/CCL17, MDC/CCL22 and their receptors CXCR3, CCR4 in the pathogenesis of bullous pemphigoid(BP). Methods The expression of CXCL9, CXCL11, CCL17, CCL22 and CXCR3 and CCR4 in 50 cases of BP lesions and 30 cases of normal skin were detected by immuno-histochemistry and optical density ( OD) . Results The ex-pression of four chemokines and their receptors positive values in 50 patients with BP were significantly higher than those in normal skin. The expression of CXCL9, CXCL11 and CXCR3 in the BP lesion and normal skin were (0.55 ±0.09) vs (0.26 ±0.08),(0.38 ±0.06) vs (0.18 ±0.04)and (0.37 ±0.04) vs (0.22 ±0.02)respec-tively, and the expression of Th2 chemokine CCL17, CCL22 and its receptor CCR4 were (0.42 ± 0.10) vs (0.25 ±0.12),(0.35 ±0.04) vs (0.21 ±0.03)and (0.42 ±0.05) vs (0.24 ±0.07),and the differences were statis-tically significant ( P ＜0.01 ) . Conclusion Th1 chemokines CXCL9, CXCL11 and Th2 chemokines CCL17, CCL22 and their receptors CXCR3, CCR4 may be associated with the pathogenesis of BP, and have a certain value in understanding the pathogenesis of BP.