999感冒灵颗粒抗呼吸道合胞病毒的初步研究

目的 探究999感冒灵颗粒在体外抗呼吸道病毒的作用.方法 采用细胞病变抑制法(CPE)及CCK-8法,观察在999感冒灵颗粒作用下,感染呼吸道合胞病毒( RSV)的HEp-2细胞的CPE变化情况以及吸光值,从而比较不同给药方式(直接灭活、抑制吸附、抑制增殖和阻断作用)及不同给药浓度下对 RSV的抑制作用.测定细胞的半数中毒浓度(TC50)、药物半数抑制浓度(IC50)以及治疗指数(TI).结果 细胞的TC50为25 mg/ml;直接灭活和抑制增殖均对RSV有明显作用( P ＜0.01 );采用直接灭活方式时,其 IC50为7.43 mg/ml,TI为3.36;采用抑制病毒增殖时,其IC50为4.42 mg/ml,TI为5.66. CCK-8 法结果显示,在阻断以及抑制吸附两种给药方式下,病毒抑制率与病毒对照组之间差异无统计学意义.结论 999 感冒灵颗粒在直接灭活以及抑制病毒增殖两种给药方式下对RSV有一定的抑制作用.

A preliminary study of 999 cold particles against respiratory syncytial virus

Objective To explore the role of 999 cold particles on the anti-respiratory virus in vitro. Methods CPE and CCK-8 were used to observe the changes of CPE and absorbance of HEp-2 cells infected with respiratory syncytial virus ( RSV) under the action of 999 cold particles, so as to compare different modes of administration ( direct inactivation, inhibition of adsorption, inhibition of proliferation and blockade) and RSV inhibition at differ-ent concentrations. Median toxic concentration ( TC50) , half inhibition concentration ( IC50) and treatment index ( TI) were measured, respectively. Results Median toxic concentration ( TC50) of 999 cold granules was 25 mg/ml. Direct inactivation and inhibition of proliferation had a significant effect on RSV (P＜0.01). In the way of di-rect inactivation, the IC50was 7.43 mg/ml and the TI was 3.36. In the method of inhibiting RSV proliferation, the IC50was 4. 42 mg/ml and the TI was 5. 66. The CCK-8 results showed that there was no significant difference be-tween the viral inhibitory rate and the viral control group in the two ways of blocking and inhibiting the adsorption. Conclusion 999 cold granule has a certain inhibitory effect on RSV by two ways: direct inactivation and inhibition of proliferation.