阿帕替尼联合放疗对HeLa细胞周期和凋亡影响的体外研究

目的 体外观察阿帕替尼(APA)联合放疗对宫颈癌 HeLa细胞周期和凋亡影响.方法 对数生长期HeLa细胞分为对照组、药物组、放疗组和联合组.流式细胞仪检测细胞周期和凋亡,分析放疗后HeLa细胞周期和凋亡变化.结果 联合组G0/G1期阻滞明显高于对照组和放疗组(P＜0. 05).联合组S期比例最低,与对照组比较差异有统计学意义(P＜0.05),与放疗组比较差异无统计学意义.联合组凋亡率高于对照组和药物组(P＜0.05).结论 APA联合放疗有明显G_0/G_1期细胞周期阻滞作用,但无明显凋亡诱导作用.

Influence of apatinib combined radiotherapy on HeLa cell cycle and apoptosis in vitro

Objective To observe the effect of apatinib (APA) combined with radiotherapy on cell cycle and apoptosis of cervical cancer HeLa cells in vitro. Methods HeLa cells in logarithmic growth phase were divided into control group, drug group, radiotherapy group and joint group. Cell cycle and apoptosis were detected by flow cytometry, and the changes of HeLa cell cycle and apoptosis after radiotherapy were analyzed. Results Retardant of G0/G1 phase for joint group was obviously higher than control and radiotherapy group (P ＜ 0. 05). S phase percentage of joint group was minimum when compared with control group (P ＜0. 05), while there was no significant difference when compared with radiotherapy group. Apoptosis rate of joint group was higher than control group and drug group (P ＜ 0. 05 ). Conclusion APA combined with radiotherapy shows significant G0/G1 phase cell cycle arrest, but no significant induction of apoptosis.