高效液相色谱波长切换法联合梯度洗脱法同时测定理气散结颗粒中6种成分的研究

目的 探讨高效液相色谱(HPLC)波长切换联合梯度洗脱法同时测定理气散结颗粒中芍药苷、柴胡皂苷a、柴胡皂苷d、延胡索乙素、去氢紫堇碱和紫堇碱6种成分的含量可行性.方法 采用Agilent TC-C18色谱柱;以乙腈(A)-0.1%冰醋酸溶液(B)为流动相,梯度洗脱;检测波长分别为230 nm(芍药苷)、210 nm(柴胡皂苷a和柴胡皂苷d)、280 nm(延胡索乙素、去氢紫堇碱和紫堇碱);流速:1.0 mL·min-1;柱温:35℃.结果 芍药苷、柴胡皂苷a、柴胡皂苷d、延胡索乙素、去氢紫堇碱和紫堇碱分别在14.39~359.75 mg·L-1、1.96~49.00 mg·L-1、1.44~36.00 mg·L-1、1.36~34.00 mg·L-1、1.58~39.50 mg·L-1、2.12~53.00 mg·L-1范围内线性关系良好,r分别为0.9998、0.9995、0.9999、0.9994、0.9997、0.9999(n=6),其平均回收率分别为99.87%、97.75%、98.09%、97.46%、98.29%和96.97%,RSD分别为0.84%、1.74%、0.71%、1.39%、1.07%和1.11%.结论 该方法简便、准确、重复性好,可作为理气散结颗粒全面质量控制方法.

Simultaneous determination of the six components in the gas diapersing granulesby HPLC wavelength switching combined with gradient elution method

Objective To establish a method for the simultaneous determination of paeoniflorin ,saikosaponina,saikosaponind,tetrahydropalmatine,dehydrocorydaline and corydaline in Liqi Sanjie Granules by HPLC wavelength switching combined with gradient elution method.Methods The Agilent TC-C18 column was used,the mobile phase was acetonitrile (A) -0.1% glacial acetic acid solution (B) with gradient elution,and the detection wavelengths were 230 nm for paeoniflorin,210 nm for saikosaponina and saikosaponind ,and 280 nm for tetrahydropalmatine,dehydrocorydaline and corydaline ,respectively.The flow rate was 1.0 mL· min-1 ,and column temperature was set at 35 ℃.Results The linear relationship of paeoniflorin,saikosaponina,saikosaponind,tetrahydropalmatine,dehydrocorydaline and corydaline was good in the ranges of 14.39 -359.75 mg· L-1 (r =0.9998),1.96 -49.00 mg· L-1 (r =0.9995),1.44 -36.00 mg· L-1 (r =0.9999),1.36 -34.00 mg· L-1 (r =0.9994),1.58 -39.50 mg· L-1 (r =0.9997),2.12 -53.00 mg· L-1 (r =0.9999),respectively.The average recoveries were 99.87%,97.75%,98.09%,97.46%,98.29% and 96.97%,and RSDs were 0.84%,1.74%,0.71%,1.39%,1.07% and 1.11%.Conclusions The method is simple,accurate andwell reproducible,which can be used as quality control method for Liqi Sanjie Granules .