溃疡性结肠炎血小板中钾通道和NLRP3表达的临床意义

目的 探讨溃疡性结肠炎(UC)血小板中钾通道和 NLRP3的表达,以及与UC临床特征的关联分析.方法 收集11例正常对照组和17例UC患者临床资料.提取血小板,采用 RT-PCR、Western blot 法检测 Kca3.1、Kvl. 3 及 NL-RP3的表达水平.结果 与正常对照组相比,UC患者血小板中Kca3. 1、Kvl.3及NLRP3 mRNA表达量均明显增加(t=-6.067、-4.991、-18.981,P<0.05),与 UC 严重程度无明显相关.UC患者血小板中Kca3. 1、Kvl. 3及NLRP3蛋白表达量均明显高于对照组,差异有统计学意义(t =-9. 627、-7. 39、-7. 821,P< 0. 05),与 UC 严重程度无明显相关.UC患者血小板中Kca3. 1与NLRP3蛋白表达水平明显相关(r =0. 877,P<0.05),Kvl.3与NLRP3蛋白表达水平有一定的相关性,但差异无统计学意义.UC患者血小板中Kca3.1、Kvl.3及NLRP3蛋白表达与患者临床指标红细胞沉降率、C反应蛋白及Mayo评分均不相关.结论 UC患者血小板中NLRP3与钾通道表达增高,两者具有一定相关.

Clinical significance of potassium channel and NLRP3 expression in platelets of ulcerative colitis

Objective To investigate the expression of potassium channels and NLRP3 in platelets of ulcerative colitis (UC), and to analyze the correlation with UC. Methods Clinical data were collected from 11 normal controls and 17 patients with UC. The levels of platelet Kca3. 1, Kvl. 3 and NLRP3 were detected by RT-PCR and Western blot after platelet extraction. Results Compared with control group, the expression of Kca3. 1, Kvl. 3 and NLRP3 in platelets from the UC patients were increased significantly at the mRNA level (t =-6. 067, 4. 991,-18. 981, P＜0. 05), which were not related to the severity of UC. The expression of Kca3. 1, Kvl. 3 and NLRP3 protein in platelets of UC patients was significantly higher than those of the control group (t =-9. 627,-7. 39,-7. 821, P＜0.05), and there was no significant correlation between their expression and the severity of UC. Correlation analysis showed that the expression of Kca3. 1 and NLRP3 protein in platelets of UC was significantly correlated (r=0. 877, P＜0. 05). There was a general correlation between Kv3. 1 and NLRP3 expression at protein level, but the difference was not statistically significant. The expression of Kca3. 1, Kvl. 3 and NLRP3 proteins in platelets of UC were not correlated with erythrocyte sedimentation rate, C-reactive protein and Mayo scores. Conclusion The expression of NLRP3 and potassium channels in platelets of UC patients is increased, and there is a certain correlation between these two index.