Interleukin-1 activates phospholipase A2 in human synovial cells |
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| Authors: | S C Gilman J Chang P R Zeigler J Uhl E Mochan |
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| Affiliation: | Division of Experimental Therapeutics, Wyeth Laboratories, Philadelphia, PA 19101. |
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| Abstract: | Interleukin-1 (IL-1) treatment of synovial cells from rheumatoid arthritis and osteoarthritis patients resulted in a dose-dependent secretion of phospholipase A2 (PLA2). IL-1 also stimulated prostaglandin E2 and plasminogen activator synthesis, in parallel with PLA2 activation; all 3 were detectable within 6 hours of IL-1 treatment and peaked by 24 hours. Synovial cell PLA2 required calcium (5 mM) and a neutral pH (7.5) for maximal activity and appears similar to the PLA2 in synovial fluid, which has been described previously. We conclude that PLA2 can be induced by IL-1, and its secretion may contribute significantly to the inflammatory actions of IL-1. |
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