|
|||||
|
|
A monoclonal antibody against an epitope common to HLA-B locus antigens |
|
| Authors: | R. Billing K. Lucero |
| Affiliation: | From the Department of Surgery, UCLA School of Medicine, University of California, Los Angeles, CA, USA |
| Abstract: | A monoclonal antibody G4 that appears to be directed against a determinant common to akl HLA-B locus antigens is described. This antibody reacted with a large panel of B and T lymphocytes and cell lines, but it did not react with two lines that do not serologically express HLA antigens (Daudi and K562) and two lines that expressed A-locus but not B-locus antigens (8402 and HPBMLT). The F(ab′)2 fragment of G4 blocked B-locus but not A-locus HLA alloantisera. By immunoprecipitation and SDS-polyacrylamide gel electrophoresis G4 reacted with a dimer consisting of a heavy chain of 44,000 daltons and a light chain of 12,7000 daltons. |
| Keywords: | AML acute myelocytic leukemia SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis HAT hypoxanthine/aminopterin/thymidine |
| 本文献已被 ScienceDirect 等数据库收录！ | |