| 川芎嗪对心肌细胞缺氧/复氧损伤的保护作用 |
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| 引用本文： | 周雅萍,刘强.川芎嗪对心肌细胞缺氧/复氧损伤的保护作用[J].实用临床医学(江西),2011,12(2):5-7,13. |
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| 作者姓名： | 周雅萍 刘强 |
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| 作者单位： | 南昌大学第二附属医院药剂科,南昌,330006 |
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| 摘 要： | 目的 研究川芎嗪对培养的SD乳鼠心肌细胞缺氧/复氧(H/R)损伤的保护作用及其机制.方法 将原代培养的SD乳鼠心肌细胞按随机数字表法分为5组,分别为正常组、H/R损伤模型组和H/R+川芎嗪给药组(川芎嗪浓度分别为50、100、150 μg·mL-1),每组10孔.测定各组心肌细胞超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及乳酸脱氢酶(LDH)活性.结果 与H/R损伤模型组相比,川芎嗪给药组可明显提高SOD活性,降低MDA含量、LDH活性,差异均有统计学意义(P＜0.05).结论 川芎嗪对体外培养心肌细胞模拟H/R损伤模型有明显的保护作用,其保护机制可能与川芎嗪抑制自由基生成或清除自由基有关.
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| 关 键 词： | 川芎嗪 心肌细胞 保护作用 缺氧/复氧损伤 动物 实验 大鼠 |
Protective Effects of Ligustrazine on Hypoxia/Reoxygenation Injury in Cardiomyocytes |
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| ZHOU Ya-ping,LIU Qiang.Protective Effects of Ligustrazine on Hypoxia/Reoxygenation Injury in Cardiomyocytes[J].Practical Clinical Medicine,2011,12(2):5-7,13. |
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| Authors: | ZHOU Ya-ping LIU Qiang |
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| Institution: | ZHOU Ya-ping,LIU Qiang(Department of Pharmacy,the Second Affiliated Hospital of Nanchang University,Nanchang 330006,China) |
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| Abstract: | Objective To investigate the protective effects of ligustrazine on hypoxia/reoxygenation injury and its mechanisms of action in cultured SD neonatal rat cardiomyocytes.Methods The primary cultured cardiomyocytes were randomly divided into five groups：normal group,hypoxia/reoxygenation model group,50 μg·mL-1ligustrazine group,100 μg·mL-1ligustrazine group and 150 μg·mL-1ligustrazine group,with 10 wells in each group.Superoxide dismutase（SOD） activity,malondialdehyde（MDA） concentrations and lactate dehydrogenase（LDH） content were measured.Results Ligustrazine significantly induced a increase in SOD activity and a decrease in MDA concentrations and LDH content,as compared to hypoxia/reoxygenation model group（P0.05）.Conclusion Ligustrazine may protect cultured neonatal rat cardiomyocytes from hypoxia/reoxygenation injury through a mechanism involving inhibiting and scavenging free radicals. |
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| Keywords: | ligustrazine cardiomyocytes protective effects hypoxia/reoxygenation injury animals laboratory rats |
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