RhD血型基因定型在新生儿溶血病产前诊断的应用

目的 检测血液RhD血型基因型并应用于新生儿溶血病(HDN)的产前诊断。方法 采用聚合酶链反应-序列特异性引物(PCR-SSP)方法，根据PCR产物的强弱判定RhD基因型。结果36名个体血液样本中，10名为RhD-／RhD-纯合子，3名为RhD ／RhD-杂合子，23名为RhD ／RhD-纯合子。结论 该方法可以准确检测出RhD血型基因型，并可用于RhD血型不合引起HDN的产前诊断。

Genotyping of RhD blood group for application in prenatal diagnosis of HDN

Objective To develop a molecular biological method for genotyping of RhD blood group and its application in prenatal diagnosis of haemolytic disease of newborn (HDN).Methods A polymerase chain reaction with sequence-specific primers (PCR-SSP) assay was designed to define RhD blood group.RhD genotyping was determined according to the intensity of PCR products.Results Among 36 samples,10 were RhD-/ RhD-homozygous RhD-negative,3 were RhD /RhD-heterozygote RhD-positive and 23 were RhD /RhD homozygous RhD-positive.Conclusions PCR-SSP is a simple and rapid method for determinion of RhD genotyping and will be very useful in prenatal diagnosis of HDN resulting from anti-D.