| 基因重组人睫状神经营养因子单克隆抗体的制备及初步鉴定 |
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| 引用本文： | 张亚莉,苏瑾,车小燕,林来兴妹,周俊岭,赵青,王小宁.基因重组人睫状神经营养因子单克隆抗体的制备及初步鉴定[J].南方医科大学学报,1999,19(6):520-522. |
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| 作者姓名： | 张亚莉 苏瑾 车小燕 林来兴妹 周俊岭 赵青 王小宁 |
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| 作者单位： | 1. 第一军医大学分子免疫学研究所广州 510515
2. 第一军医大学中心实验室广州 510515 |
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| 摘 要： | 目的 制备基因重组人睫状神经营养因子 (rhCNTF)单克隆抗体。方法利用rhCNTF免疫BALB/C小鼠 ,取其脾细胞与小鼠NS -1骨髓瘤细胞融合 ,制备 3株抗rhCNTF的单克隆抗体 ,分别命名为CA2 、CA6 和BB11。结果 3株抗体类型均为IgG2a,免疫印迹试验证明为特异性抗CNTF的单克隆抗体 ;抗体相加试验表明 3株单抗是针对CNTF两个不同的抗原决定簇。
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| 关 键 词： | 基因重组人睫状神经营养因子 单克隆抗体 |
| 修稿时间： | 1999年6月11日 |
Preparation and identification of monoclonal antibodies against recombinant human ciliary neurotrophil factor |
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| Zhang Yali ,Su Jin ,Che Xiaoyan ,Lin Laixingmei ,Zhou Junling ,Zhao Qing ,Wang Xiaoning Institute of Molecular Immunology,Central Laboratory,First Military Medical University,Guangzhou.Preparation and identification of monoclonal antibodies against recombinant human ciliary neurotrophil factor[J].Journal of Southern Medical University,1999,19(6):520-522. |
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| Authors: | Zhang Yali Su Jin Che Xiaoyan Lin Laixingmei Zhou Junling Zhao Qing Wang Xiaoning Institute of Molecular Immunology Central Laboratory First Military Medical University Guangzhou |
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| Institution: | Zhang Yali 1,Su Jin 1,Che Xiaoyan 1,Lin Laixingmei 1,Zhou Junling 2,Zhao Qing 2,Wang Xiaoning 1 1Institute of Molecular Immunology,2Central Laboratory,First Military Medical University,Guangzhou,510515 |
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| Abstract: | Objective To produce monoelonal antibodies against recombinant human ciliary neurotrophic factor (rhCNTF) Methods rhCNTF was used to immune BALB/C mouse, the spleen cells from which were fused with murine NS-1 myeloma cells 3 monoclonal antibodies against rhCNTF were produced from the hybridoma, named CA2, CA6 and BB 11 respectively Results All the 3 antibodies were in the IgG2a subclass and proved to be specific antibodies against CNTF By Western blot experiment, McAb additivity test showed that the 3 antibodies recognize 2 different epitopes |
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| Keywords: | rhcNTF McAb |
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