结直肠癌血清标志物在不同条件下的稳定性研究 |
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引用本文: | 吴洪巧,郏雁飞,王庆西,张涌,张振.结直肠癌血清标志物在不同条件下的稳定性研究[J].预防医学文献信息,2010(12):1116-1117. |
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作者姓名: | 吴洪巧 郏雁飞 王庆西 张涌 张振 |
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作者单位: | [1]山东大学附属济南市中心医院,山东济南250013 [2]山东大学医学院2007级一班,山东济南250012 |
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摘 要: | 目的]观察不同保存条件对血清CEA、CA199、AFP浓度的影响。方法]取结直肠癌患者血清样品,在取血当时、冷藏(4±1)℃保存3周,以及室温(28±3)℃保存的条件下,采用酶免疫分析法(EIA),测定血清中CEA、CA199、AFP的浓度并进行比较,考察其稳定性。结果]冰箱冷藏保存1、2、3周及室温保存3 d的血清测定结果均与取血当时测定结果无统计学差异(P〉0.05)。室温保存1、2周的血清测定结果明显高于取血当时测定结果(P〈0.05)。结论]CEA、CA199、AFP血清样品在冷藏条件下可稳定保存3周,室温下可稳定保存3 d。
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关 键 词: | CEA CA199 AFP 冷藏 室温 稳定性 |
Effect of Sample Storage on Stability of Colorectal Cancer Serum Markers |
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Institution: | WU Hong-qiao,JIA Yan-fei,WANG Qing-xi,et al.(Ji'nan Central Hospital,Affiliated to Shandong University,J'inan,250013,Shandong,China) |
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Abstract: | Objective] To investigate the effects on serum concentrations of CEA、CA199,and AFP with different storage requirements.Methods]The serum samples from colorectal cancer patients were stored in refrigeration(4±1) ℃ for l week,2 weeks,and 3 weeks,or at normal temperature(28±3) ℃ for 3 days,1 week and 2 weeks,then these samples were determined by enzyme immunoassay(EIA) and the results were compared with the new ones.Results]There were no significant differences between the samples stored in refrigeration(4±1) ℃ for l week,2 weeks,and 3 weeks and the samples stored at normal temperature for 3 days.Serum results stored at normal temperature for 1 week and 2 weeks were significantly higher than those at the collection time.Conclusion]Serum samples of CEA,CA199 and AFP can be stored stably in refrigeration for 3 weeks or at normal temperature for 3 days. |
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Keywords: | CEA CA199 AFP Refrigeration Normal temperature Stability |
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