| 靶向VEGF基因的siRNA联合树突状细胞介导的CTL对MCF-7细胞作用的研究 |
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| 引用本文： | 王海燕,李延年,董永,孙波,张树超,葛银林. 靶向VEGF基因的siRNA联合树突状细胞介导的CTL对MCF-7细胞作用的研究[J]. 中国输血杂志, 2010, 23(2) |
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| 作者姓名： | 王海燕 李延年 董永 孙波 张树超 葛银林 |
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| 作者单位： | 1. 青岛大学医学院附属医院,输血科,山东,青岛,266003
2. 青岛大学医学院,生化教研室 |
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| 基金项目： | 青岛市科技局科技计划项目(编号:07—2-1—7-nsh) |
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| 摘 要： | 目的探讨靶向血管内皮细胞生长因子(VEGF)基因的小干扰RNA(siRNA)联合负载肿瘤抗原的树突状细胞(DC)致敏的细胞毒性T淋巴细胞(CTL)的联合抗瘤作用。方法体外应用靶向VEGF基因最佳转染浓度(100nmol/L,增加浓度不再提高沉默作用)的siRNA联合负载人乳腺癌细胞(MCF-7细胞)抗原的DC介导的CTL(siRNAVEGF+CTL组)共同作用于MCF-7细胞,同时设立空白对照组和空白+CTL组(只加无血清无抗生素培养基);脂质体组和脂质体+CTL组(只加LipofectamineTM2000);siRNAVEGF-组(100nmol/LsiRNA);siRNASCR组和siR-NASCR+CTL组(100nmol/LsiRNASCR),每组做6个平行孔,四甲基偶氮唑蓝(MTT)法检测siRNAVEGF+CTL组和各对照组肿瘤杀伤活性(n=6),Hoechst33258核染色观察细胞的凋亡(n=3)。结果siRNAVEGF+CTL组、siR-NAVEGF-组siRNASCRCTL组肿瘤杀伤活性分别为99.37%,51.17%和43.94%,siRNAVEGF+CTL组与对照组比较可明显杀伤肿瘤细胞,瘤细胞几乎完全溶解,Hoechst33258显示细胞核呈明显的细胞凋亡改变。结论体外实验显示靶向VEGF的siRNA联合负载肿瘤抗原DC致敏的CTL能有效抑制乳腺癌MCF-7细胞的生长,二者的联合应用抗瘤效果显著。
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| 关 键 词： | RNA干扰 VEGF 树突状细胞 MCF-7细胞 |
Effects of siRNA directed against VEGF gene and CTL mediated by DCs loaded tumor antigen on MCF-7 Breast Cancer cells |
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| WANG Haiyan,LI Yannian,Dong Yong,Ge Yinlin,et al.. Effects of siRNA directed against VEGF gene and CTL mediated by DCs loaded tumor antigen on MCF-7 Breast Cancer cells[J]. Chinese Journal of Blood Transfusion, 2010, 23(2) |
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| Authors: | WANG Haiyan LI Yannian Dong Yong Ge Yinlin et al. |
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| Affiliation: | WANG Haiyan1,LI Yannian1,Dong Yong1,Ge Yinlin2,et al. 1.Department of Transfusion,the Affiliated hospital of Medical College,Qingdao University,Qingdao,266003,China,2.Department of Biochemistry , Molecular Biology,Medical College |
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| Abstract: | Objective To investigate the effects of siRNA directed against VEGF gene and cytotoxic T lymphocyte(CTL)mediated by DCs loaded tumor-specific antigen on MCF-7 Breast Cancer cells.Medhods In vitro small interfering RNAs(siRNAs)directed against VEGF gene were designed and transfected into MCF-7 breast cancer cells at the optimal concentration(100 nmol/L)using cationic liposome LipofectamineTM 2000,then combinted CTL mediated by DCs(siRNAVEGF+CTL group)were used to against MCF-7 breast cancer cells.At the same... |
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| Keywords: | siRNA VEGF Dendritic cells MCF-7 cells |
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