维生素C和谷胱甘肽对离体肺泡巨噬细胞超微弱发光的影响

目的　研究外源维生素 C(VC)和谷胱甘肽 (GSH)对家兔离体肺泡巨噬细胞(AM)超微弱 (自主 )发光的影响。方法　将 AM悬浮在含有 VC或 GSH的 DMEM培养介质中 ,放入一特制恒温箱的培养盒内 ,连续通入不同浓度的氧气 ,用发光仪检测培养 AM的超微弱发光。结果　浓度超过 0 .3mmol/L的 VC能明显增强有氧培养细胞超微弱发光和加速细胞死亡 ,对无氧培养细胞的影响不明显。低浓度 (0 .0 3mmol/L) VC与 GSH相同 ,能降低由高浓度 (99.1 % )氧暴露引起的细胞发光增强。结论　细胞在含氧气体中的自发氧化是产生超微弱发光的重要原因 ;高浓度 VC能促进离体细胞氧化损伤 ,GSH则有抗氧化作用。

EFFECTS OF VITAMIN C AND GLUTATHIONE ON ULTRAWEAK LUMINESCENCE OF PULMONARY ALVEOLUS MACROPHAGES IN VITRO

Objective:[WT5BZ] The effects of exogenous vitamin C (VC) and glutathione (GSH) on ultraweak spontaneous luminescence of culturing pulmonary alveolus macrophages from rabbits were studiesd. [WT5FZ]Methods:[WT5BZ] In a special thermostat, which was passed through by airs with various concentrations of oxygen, the alveolar macrophages (AMs) were cultured in DMEM medium with VC or GSH, and the spontaneous luminescence of culturing AMs was examined by a luminometer. [WT5FZ]Results:[WT5BZ] when VC in medium was over 0.3 mmol/L, it could significantly enhance the oxidative luminescence of cells exposed to O 2 and cell death was resulted. However, when the cells were exposed to air without O 2 there was no significant effect. On the contrary,the lower concentration of VC (0.03 mmol/L) as well as GSH could reduce the spontaneous luminescence of cells exposed to a high concentration (99.1%) of O 2 in air. [WT5FZ]Conclusion:[WT5BZ] The results show that the spontaneous oxidation of culturing cells is an important reason for the ultraweak luminescence. High concentration of VC can promote cellular oxidative damage in vitro, but the exogenous GSH has a protective effect against oxidization in culturing cells.