纤溶酶原激活物抑制物-1与动脉粥样硬化关系的研究

目的 通过对动脉粥样硬化（AS）兔血清和粥样硬化斑块中纤溶酶原激活物抑制物-1（PAI-1）表达的研究,探讨PAI-1在AS中的作用.方法 16只雄性大耳白兔随机分为正常饮食组和高脂饮食组,每组8只,饲养16周.两组白兔均于0周、16周取耳缘静脉血,检测血清中PAI-1 的水平;16周后处死,应用免疫组化方法检测PAI-1在主动脉粥样硬化斑块中的表达.结果 正常饮食组和高脂饮食组0周血清中PAI-1水平差异无显著性（P＞0.05）;高脂饮食组16周后血清PAI-1水平较0周显著增加（P＜0.01）;免疫组化结果显示高脂饮食组主动脉壁PAI-1 的表达明显高于对照组（P＜0.01）.结论 动脉粥样硬化的发生伴有血清和粥样斑块中PAI-1表达增加,PAI-1可能参与了AS的形成.

Study on relationship of plasminogen activator inhibitor -1 and atherosclerosis in rabbits

Objective To study the expression of plasminogen activator inhibitor-1(PAI-1) of the blood serum and plaques in atherosclerotic rabbits in order to explore the effect of PAI-1 on atherosclerosis (AS).Methods 16 male white rabbits were randomly divided into normal diet group and high cholesterol diet group (n=8 for each).The rabbits were fed for 16 weeks.Blood was collected from the marginal vein of the ear in 0 and 16 weeks respectively in two groups.Concentration of PAI-1 was measured; After 16 weeks,the rabbits were sacrificed to investigate the expression of PAI-1 in aortic atherosclerosis by immunohistochemistry analysis.Results There were not obvious differences of PAI-1 in the normal diet group and the high-cholesterol diet group at 0 week (P>0.05),obvious increment of PAI-1 in the high-cholestrol diet group after 16 weeks than 0 week (P<0.01); the higher expression of PAI-1 in cholesterol diet group was higher than that in control group (P<0.01).Conclusion PAI-1 expression is increased in blood serum and plaque ,which is correlated with atherosclerosis,suggesting that PAI maybe involved in the formation of AS.