宫颈癌治疗性多肽疫苗的合成及其诱导细胞毒性T淋巴细胞效应的研究

目的：设计并合成宫颈癌治疗性多肽疫苗，并对其免疫学效应进行研究。方法：以芴甲氧羰基固相法合成多肽，用高效液相色谱分析多肽的纯度，对所合成的多肽采用质谱进行鉴定，用标准^51Cr释放试验检测各肽在HLA—A2转基因鼠中诱导的表位特异性细胞毒性T淋巴细胞活性。结果：合成的多肽经纯化后纯度分别为86．5％、81．8％和98，5％，质谱分析各肽的相对分子质量分别为3121、2580和815，与理论值相符。其在HLA—A2转基因鼠中可诱导出强的表位特异性细胞毒性T淋巴细胞活性。结论：合成的多肽为目标肽，将辅助性T细胞表位及脂类分子构建于分子内部可提高细胞毒性T淋巴细胞表位肽的免疫原性。

Synthesis and cytotoxic T lymphocyte response in vivo of therapeutic vaccine against cervical cancer

AIM:To design and synthesize the therapeutic vaccine against human cervical cancer and study its immunological effect.METHODS:The peptides were synthesized with solid phase strategies,purified with reverse phase HPLC and identified with mass spectrometry.Their immunological effects inducing(CTL)response were investigated in HLA-A2 transgenic mice,and the activity of specific CTL was measured by using a standard 4 h51Cr release assay.RESULTS:The purities of peptides were 86.5%,81.8% and 98.5%,respectively.Their molecular weights were 3 121,2 580 and 815 respectively,which was the same as expected molecular mass.The therapeutic vaccine could induce strong specific CTL activity in HLA-A2 transgenic mice.CONCLUSION:The synthetical products are the target peptides.The combination of CTL and T helper epitope and lipid molecule can remarkably improve the immunogenicity of CTL peptide.