急性酒精过量对SD大鼠氧化应激的影响

目的:定量测定与评价急性过量酒精灌胃对大鼠氧化应激的影响。方法:将成年雄性SD大鼠随机分为两组,每组10只。对照组给予大鼠灌胃纯净水;实验组按7.6 ml/kg给大鼠灌胃50%酒精,每天2次,连续3d,制作酒精急性氧化损伤模型。测定大鼠血液中丙二醛(malondialdehyde,MDA)的含量,总抗氧化力(total antioxidant force,T-AOC)的改变和超氧化物歧化酶(superoxide dismutase,T-SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽硫转移酶(glutathione sulfur transferase,GST)等活性的变化。结果:与对照组相比,实验组大鼠血清中MDA含量明显升高(P0.05);而T-SOD活性无明显变化(P0.05),但CAT、GPx、GST活性和T-AOC活力均明显升高(P0.05)。结论:过量酒精的摄入可以引起机体氧化应激,表现为脂质过氧化产物增多;抗氧化酶活性诱导性升高,提示只要不损伤机体抗氧化代偿机制,机体仍能够维持氧化-抗氧化体系的平衡。

Oxidative stress in SD rats induced by excessive alcohol

OBJECTIVE:To study the determination and evaluation of intragastric administration of alcohol on antioxidative stress of rats.METHODS:The rats were gavaged with 50%alcohol(7.6 ml/kg) to establish the alcohol acute damage model,and then to measure the malondialdehyde(MDA) content,total antioxidant force(T-AOC)ability and superoxide dismutase(T-SOD) and catalase(CAT) activities in blood.RESULTS:The serum MDA content of experimental group was significantly higher than that in the control group(P<0.05).T-SOD was not significantly changed in either group.The aciticities of antioxidation enzyme such as CAT,GPx and GST in the experimental group were higher compared with negative control(P<0.05).T-AOC stayed the same.CONCLUSION:Oxidative stress could happen in over-drinking with more LPO production.At the same time the activities of antioxidative eyzmes were increased.The organism could keep the balance if the mechanism of oxidation-reduction remains intact.