川芎汤吸收入人血清中阿魏酸HPLC法测定及其药动学研究

目的本文对以往建立的人血清中阿魏酸浓度测定的HPLC法加以改进,并进行阿魏酸的临床药动学研究.方法以香豆素为内标,甲醇-水-醋酸(4059.70.3,v/v)为流动相,Diamonsil(TM)色谱柱(150mm×4.6mm,5μm)为固定相,流速1.0ml/min.以二极管阵列检测器测定并提取320nm波长下的数据,通过三维图观察其紫外吸收以定性,用沸水浴法处理血清样品.结果阿魏酸和内标分离完全,阿魏酸在40.16～8032ng/ml范围内线性关系良好(r=0.9975),日内和日间变异系数均<10%,平均回收率为100.3%,最低检测限为2.51ng,最低检测浓度为25.1ng/ml.PK属于开放性二室模型.结论此法简单、快速、灵敏而准确,适用于人血清阿魏酸的测定.

Determination of Ferulic Acid Absorbed into Human Serum from Oral Decoction of Rhizoma Chuanxiong and Study on Pharmacokinetic of Ferulic Acid

OBJECTIVE: To improve the HPLC method for determining ferulic acid(FA) in the human serum and to research FA clinical pharmacokinetics. METHOD: Serum concentrations of FA were determined by HPLC using methanol-water-acetic acid (40:59.7:0.3, v/v) as the mobile phase and the column packed with ODS (150 mm x 4.6 mm, 5 microns) as a fixed phase and the flow rate was 1.0 ml/min. FA was detected at 320 nm wave length. The internal standard was coumarin. The serum samples were treated by the water-boiling method. Peak of FA in serum was recognized by photodiode-array detection. RESULT: FA and internal standard were separated completely under the condition described as above. FA was linear in the range of (40.16-8032 ng/ml) (r = 0.9975). The CV was less than 10%, the average recovery was 100.3%, and the limit concentration in serum was 25.1 ng/ml. PK was in accordance with open bicameral model. CONCLUSION: This method can be applied to clinical pharmacokinetic study of FA.