| 两步超速离心法快速分离大量血浆极低密度脂蛋白及低密度脂蛋白 |
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| 引用本文： | 王淳本,宗义强,吴万生,冯友梅,邓耀祖,冯宗忱. 两步超速离心法快速分离大量血浆极低密度脂蛋白及低密度脂蛋白[J]. 华中科技大学学报(医学版), 1995, 0(3) |
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| 作者姓名： | 王淳本 宗义强 吴万生 冯友梅 邓耀祖 冯宗忱 |
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| 作者单位： | 同济医科大学基础医学院生物化学教研室 |
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| 摘 要： | 本法先使血浆中的脂蛋白快速富集浓缩，再将脂蛋白各组份分离纯化。即具备序列漂浮法分离样品量大的长处，又具有密度梯度法离心时间短的优点。使用ＢＥＣＫＭＡＮＬ８－８０Ｍ型超速离心机，Ｔｙｐｅ７０Ｔｉ转头，分离２８０ｍｌ血浆只需超速离心５ｈ。所得极低密度脂蛋白（ＶＬＤＬ）及低密度脂蛋白（ＬＤＬ）经琼脂糖电泳、聚丙烯酰胺电泳（ＰＡＧＥ）及载脂蛋白ＳＤＳ－聚丙烯酰胺电泳（ＳＤＳ－ＰＡＧＥ）鉴定纯度良好。本文为超速离心分离制备大量血浆ＶＬＤＬ、ＬＤＬ提供了一种快速、经济的新方法。
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| 关 键 词： | 超速离心法；脂蛋白类．极低密度；脂蛋白类．低密度 |
Rapid Isolation of Large Amount of Plasma VLDL and LDL by a Two Step ultracentrifugation |
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| Wang Chunben,Zong Yiqiang,Wu Wansheng,et al. Rapid Isolation of Large Amount of Plasma VLDL and LDL by a Two Step ultracentrifugation[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1995, 0(3) |
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| Authors: | Wang Chunben Zong Yiqiang Wu Wansheng et al |
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| Abstract: | A rapid method to obtain large amount of VLDL and LDL by ultracentrifugation is described.This method combines the advantages of both sequential flotation ultracentrifugation and density gradient ultracentrifugation.It can process a large volume of plasma in a short time.The purity of isolated VLDL and LDL was confirmed by the lipoprotein electrophoresis on agarose gel and PAGE and by the apolipoprotein electrophoresis on SDS-PAGE.This rapid,economical method is of great value in practical application. |
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| Keywords: | ultracentrifugation lipoproteins VLDL lipoproteins LDL |
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