丁香胃灵合剂对慢性萎缩性胃炎大鼠SS,Bcl-2,Bax的影响

目的 探讨丁香胃灵合剂( CWLM)对慢性萎缩性胃炎(CAG)大鼠生长抑素(SS)、Bcl-2、Bax的影响及机制.方法 将60只健康Wistar大鼠随机分为5组,即正常组,CAG组,CWLM低、中、高剂量组,每组12只.采用水杨酸钠联合氨水建立大鼠CAG模型,造模2月后开始给予药物干预,CWLM按16,8,4 g/(...

Effects of Clove Weiling Mixture on SS, Bcl-2 and Bax in rats with chronic atrophic gastritis

Objective To explore the effects and mechanism of Clove Weiling Mixture(CWLM) on somatostatin(SS),Bcl-2 and Bax in rats with chronic atrophic gastritis(CAG).Methods 60 Wistar rats were randomly divided into normal group,CAG group,CWLM low-dose group,CWLM middle-dose group and CWLM high-dose group,each group had 12 rats.CAG model was established in rats by sodium salicylate and ammonia water.Drug intervention began after being made model 2 months.The rats were treated with 16,8,4 g/(kg·d) dose of CWLM.After 50 days,all rats were anesthetized to measure the content of SS in blood serum by ELISA method,and the expression of Bcl-2 and Bax in gastric mucosa by immunohistochemistry method.Results Compared with normal group,the content of SS in blood serum and the positive expression rate of Bcl-2 in gastric mucosa in CAG model were significantly increased,but the positive expression rate of Bax was significantly decreased(P<0.01).Compared with CAG group,the content of SS of CWLM middle-dose group and CWLM high-dose group were significantly decreased and the positive expression rate of Bax was significantly increased,the positive expression rate of Bcl-2 of CWLM high-dose group was significantly decreased(P<0.01),and the effects of CWLM high-dose group were most significant(P<0.01 or P<0.05).Conclusion CWLM has better curative effects on CAG rat model,and decreasing the content of SS in blood serum and the expression of Bcl-2 in gastric mucosa,increasing the expression of Bax may be one of mechanisms of its action.