重组人生长激素对大鼠肠道缺血再灌注后肠壁组织T淋巴细胞亚群和浆细胞凋亡的影响

目的探讨肠道屏障功能损害时应用重组人生长激素（rhGH）对肠道免疫屏障中肠黏膜及固有层内T淋巴细胞亚群和浆细胞凋亡的影响。方法将60只Wistar大鼠雌雄各半随机分为实验组和对照组，按实验天数又各分为3组，每组10只，以肠缺血再灌注模型造成肠道屏障功能损害的病理现象，实验组给予rhGH每日（1．33U／kg体重），采用免疫组织化学双染法观察回肠末端黏膜固有层内CD8^＋、CD4^＋、CD3^＋T淋巴细胞数及IgA浆细胞的凋亡数量变化。结果（1）对照组CD3^＋T淋巴细胞及IgA浆细胞的凋亡数量第6天明显高于第2、4天。差异有统计学意义（P〈0．01）。（2）实验组CD8^＋、CD4^＋T淋巴细胞的凋亡数量第6天明显低于第4天，差异有统计学意义（P〈0．01）。（3）实验组与对照组相比第2、4、6天IgA浆细胞的凋亡数量均明显减少。差异有统计学意义（P〈0．01）。实验组与对照组相比大鼠第2天CD8^＋、CD4^＋、CD3^＋T淋巴细胞的凋亡数量均减少，差异有统计学意义（P〈0．05，P〈0．01）。结论肠道屏障功能损害时应用重组人生长激素能够减少肠道免疫细胞的凋亡数量，对IgA浆细胞作用最明显。可能的作用时间在第6天。

Effect of recombinant human growth hormone on apoptosis of T lymphocytes and IgA plasmocytes of intestinal wall after intestinal ischemia-reperfusion injury

Objective To explore the influence of recombinant human growth hormone (rhGH) on apoptosis of T lymphocyte subgroubs and IgA plasmocytes of intestinal wall of the gut immune barrier during the gut barrier injury. Methods Sixty Wistar rats were divided into 2 groups randomly:experimental group receiving rhGH and control group. Each group was divided into 3 subgroups (2, 4, 6 days, respectively) . The rats in experimental group were injected with rhGH (1.33 U/kg every day) on postmodel every day. The changes of apoptosis of CD8+ , CD4+ , CD3+ T lymphocytes and IgA plasmocytes of ileal mucosal were observed by immuohistochemical method. Results (1) On the 6th day the apoptosis of CD3+ T cells and IgA plasmocytes in the control group was markedly increased as compared with that on the 2nd day and the 4th day (P<0.01). (2) On the 6th day the apoptosis of CD8+ and CD4+ T cells in the experimental group was decreased as compared with that on the 4th day (P<0.01).(3) On the 2nd, 4th and 6th day the apoptosis of IgA plasmocytes was gradually decreased and there was significant difference between between the control and the experimental groups ( P < 0. 01) . On the 2nd day the apoptosis of CD8+ , CD4+ , CD3+ T lymphocytes was gradually decreased and there was significant difference between the control and the experimental groups (P<0.05, P<0.01). Conclusion During the period of gut barrier injury, apoptosis of T lymphocyte subgroubs and IgA plasmocytes of intestinal mucosal and laminapropria of the gut immune barrier is alleviated by using rhGH, especially of IgA plasmocytes. The probable time of action may be on the 6th day.