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| 大鼠胰岛移植物制备与异种移植 | |
| 引用本文： | 周茂华,陈东明,唐军民,夏兆骥.大鼠胰岛移植物制备与异种移植[J].中华器官移植杂志,1998,19(4):200-201. |
| 作者姓名： | 周茂华 陈东明 唐军民 夏兆骥 |
| 作者单位： | 1. 中国武警医学院外科教研室,天津,300162 2. 北京医科大学第三临床医学院成形外科研究中心 3. 基础医学院组织学与胚胎学系 |
| 摘 要： | 增加胰岛收获量，提高胰岛纯度一直是胰岛移植中面临的重要问题，本实验经胰管注射胶原酶，胰静止消化分离成年大鼠胰岛纯度一直是胰岛葡聚糖离心纯化。纯化后胰岛收获量为６１０－８２０个／胰纯度达９２％；胰岛形态结构完整，内分泌细胞超微结构保持良好，对葡萄糖刺激反应胰岛素释放量是基本分泌水平的８倍；异种移植可逆转实验性糖尿病小鼠的高血糖达一周。 |
| 关 键 词： | 胰岛移植 分离 纯化 异种移植 移植物 |
Preparation of rat islet transplant and xenotransplantation |
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| Zhou Maohua,Chen Dongming,Tang Junmin,et al..Preparation of rat islet transplant and xenotransplantation[J].Chinese Journal of Organ Transplantation,1998,19(4):200-201. | |
| Authors: | Zhou Maohua Chen Dongming Tang Junmin |
| Institution: | Zhou Maohua,Chen Dongming,Tang Junmin,et al. Department of Surgery,Medical College of Chinese People's Armed Police Forces,Tian Jin 300162 |
| Abstract: | Rat islets were isolated with in situ duetal collagenase distention, stationary diges-tion and dextran gradient separation.After purification, about 610～820 islets/pancreas were ob-tained, with an average purity of 92%islets.The isolated islets demonstrated a well-preserved en-docrine cell ultrastrueture, responded to glucose stimulation with an eight-fold increase of insulin secretion over basal levels, and temporarily reversed experimemal diabetes in mice.This method produced a high yield of viable purified rat islets. |
| Keywords: | Islet Separation Purification Dextran Xenotransplantation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |